Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase.

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Abstract Text:

Takahiro Suzuki,Shinsuke Usuda,Hiroshi Ichinose,Satoshi Inouye,

Using photon counting and charge-coupled device (CCD) cameras, we have applied the method of real-time bioluminescence imaging to investigate protein trafficking in mammalian cells. In the living cells of Chinese hamster ovary and PC12D cells, exocytotic secretion of protein and protein targeting on the cell surface were visualized using the secreted Gaussia luciferase (GLase) as a reporter protein in a minute. After incubation of the cells with luciferin (coelenterazine) for 10min, luciferin was imported into the cells and the vesicle transport network in the cells could be shown by luminescence images of GLase activity. Further, we demonstrate that GLase with a heterologous signal peptide sequence is targeted to the cell surface in neuronally differentiated PC12D cells and luminescence signals could be detected in a few seconds.

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Publishing Authors By Initials

T Suzuki,S Usuda,H Ichinose,S Inouye,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

PUBMED ID PMID:

MEDLINE DATE:

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEBS letters

VOLUME: 581

Page Numbers: 4551-6

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 27

MONTH: 08

YEAR: 2007

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 155157

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: secretion

Chemical & Substance for Abstract: Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase.

AFFILIATION: Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: FEBS Lett

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase Related Publications

• A new method for the extracellular production of recombinant thermolysin by co-expressing the mature sequence and pro-sequence in Escherichia coli.
• Artificial DNAs based on alkynyl C-nucleosides as a superior scaffold for homo- and heteroexcimer emissions.
• Autotomic behavior of the propeptide in propeptide-mediated folding of prosubtilisin E.
• Bacterial bioreactors for high yield production of recombinant protein.
• Cerebral perfusion changes in older delirious patients using 99mTc HMPAO SPECT.
• Characteristics associated with delirium in older patients in a medical intensive care unit.
• Depressive symptoms and the risk of incident delirium in older hospitalized adults.
• Dissection of 16S rRNA methyltransferase (KsgA) function in Escherichia coli.
• Does educational attainment contribute to risk for delirium? A potential role for cognitive reserve.
• Effects of site-directed mutagenesis of the surface residues Gln128 and Gln225 of thermolysin on its catalytic activity.
• Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli.
• Identification of intracellular localization signals and of mechanisms underlining the nucleocytoplasmic shuttling of human aryl hydrocarbon receptor repressor.
• Improving the activity and stability of thermolysin by site-directed mutagenesis.
• Kinetic analysis of the activation-and-inhibition dual effects of cobalt ion on thermolysin activity.
• Molecular mechanism of the inhibitory effect of cobalt ion on thermolysin activity and the suppressive effect of calcium ion on the cobalt ion-dependent inactivation of thermolysin.
• NBK/BIK antagonizes MCL-1 and BCL-XL and activates BAK-mediated apoptosis in response to protein synthesis inhibition.
• Orthologous gene of beetle luciferase in non-luminous click beetle, Agrypnus binodulus (Elateridae), encodes a fatty acyl-CoA synthetase.
• Photooxygenation of alkynylperylenes. Formation of dibenzo[jk,mn]phenanthrene-4,5-diones.
• Real-time bioluminescence imaging of a protein secretory pathway in living mammalian cells using Gaussia luciferase.
• Recognizing delirium superimposed on dementia: assessing nurses' knowledge using case vignettes.
• Serum biomarkers for delirium.
• Structural and functional studies of the HAMP domain of EnvZ, an osmosensing transmembrane histidine kinase in Escherichia coli.
• Studies on monoterpene glucosides and related natural products.
• Study of superoxide dismutase's expression in the colon produced by azoxymethane and inositol hexaphosfate's paper, in mice.
• Temporal profile of circadian clock gene expression in a transplanted suprachiasmatic nucleus and peripheral tissues.
• The Design and Development of Tar-EnvZ Chimeric Receptors.
• The Inhibitory Effect of Aryl Hydrocarbon Receptor Repressor (AhRR) on the Growth of Human Breast Cancer MCF-7 Cells.
• The role of neuroimaging in elucidating delirium pathophysiology.
• Two New Iridoid Glucosides of Plantago asiatica.
• Use of a computer-based reminder to improve sedative-hypnotic prescribing in older hospitalized patients.