Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells.

Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells. Research Abstract Details 

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Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells. Abstract Text:

Zi-Bing Wang,Yu-Qing Liu,Ying Zhang,Yan Li,Xiao-Xia An,Han Xu,Ying Guo,Wei Jin,Zhu-Jun Jiang,Yu-Fang Cui,Zi-Bing Wang,Yu-Qing Liu,Ying Zhang,Yan Li,Xiao-Xia An,Han Xu,Ying Guo,Wei Jin,Zhu-Jun Jiang,Yu-Fang Cui,

The aim of the study was to investigate the sensitivity of AHH-1 human lymphoblastoid cells to radiation and its relevance to intracellular events, specifically alteration in cellular energy-producing systems. AHH-1 human lymphoblastoid cells were irradiated with 6 Gy of gamma radiation, and then were collected at the indicated time points. Parallel studies were conducted to assess the effects of radiation on the cell proliferation and apoptotic index. Mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) production were monitored. A marked decrease of cell viability was observed as early as 12 h postirradiation and fraction of apoptotic cells was highest at 24 h. Intracellular ROS generation measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) appeared to be highest as early as 30 min postirradiation and resumed to normal level at 6 h. Unexpectedly, the fluorescence intensity of Rhodamine 123 for measuring MMP did not change during the first 3h after radiation and exhibited an aberrant increase at 6 h. The results suggest that AHH-1 cells are sensitive to radiation-induced apoptosis and ROS generation is an early phase in the apoptosis process. Moreover, the results might cast doubts on those studies using Rhodamine 123 which hypothesized that the fall in MMP is one of the early events of apoptosis.

Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells. Publishing Authors By Initials

ZB Wang,YQ Liu,Y Zhang,Y Li,XX An,H Xu,Y Guo,W Jin,ZJ Jiang,YF Cui,ZB Wang,YQ Liu,Y Zhang,Y Li,XX An,H Xu,Y Guo,W Jin,ZJ Jiang,YF Cui,

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Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cell biology international

VOLUME: 31

Page Numbers: 1353-8

Journal Abbreviation: Cell Biol. Int.

ISSN: 1065-6995

DAY: 21

MONTH: 05

YEAR: 2007

Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells. Information

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LANGUAGE: eng

NlmUniqueID: 9307129

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Grant and Affiliation Information for Reactive oxygen species, but not mitochondrial membrane potential, is associated with radiation-induced apoptosis of AHH-1 human lymphoblastoid cells.

AFFILIATION: Department of Immunology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, P.R. China.

Country: England

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MEDLINETA: Cell Biol Int

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