Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes.

Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Research Abstract Details 

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Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Abstract Text:

Ish Kumar,Helen R Josephine,R F Pratt,

The membrane-bound bacterial D-alanyl- D-alanine peptidases or penicillin-binding proteins (PBPs) catalyze the final transpeptidation reaction of bacterial cell wall biosynthesis and are the targets of beta-lactam antibiotics. Rather surprisingly, the substrate specificity of these enzymes is not well understood. In this paper, we present measurements of the reactivity of typical examples of these enzymes with peptidoglycan-mimetic beta-lactams under in vivo conditions. The minimum inhibitory concentrations of beta-lactams with Escherichia coli-specific side chains were determined against E. coli cells. Analogous measurements were made with Streptococcus pneumoniae R6. The reactivity of the relevant beta-lactams with E. coli PBPs in membrane preparations was also determined. The results show that under none of the above protocols were beta-lactams with peptidoglycan-mimetic side chains more reactive than generic analogues. This suggests that in vivo, as in vitro, these enzymes do not specifically recognize elements of peptidoglycan structure local to the reaction center. Substrate recognition must thus involve extended structure.

Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Publishing Authors By Initials

I Kumar,HR Josephine,RF Pratt,

For similar organic chemicals: amides: lactams: beta-lactams research abstracts see: organic chemicals: amides: lactams: beta-lactams research

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Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: ACS chemical biology

VOLUME: 2

Page Numbers: 620-4

Journal Abbreviation: ACS Chem. Biol.

ISSN: 1554-8937

DAY: 21

MONTH: Sep

YEAR: 2007

Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Information

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LANGUAGE: eng

NlmUniqueID: 101282906

Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Keywords Mesh Terms:

KEYWORDS: beta-Lactams

MESH TERMS: metabolism

Chemical & Substance for Abstract: Reactions of peptidoglycan-mimetic beta-lactams with penicillin-binding proteins in vivo and in membranes. Information

Substance Name: beta-Lactams

Registry Number: 0

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Country: United States

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MEDLINETA: ACS Chem Biol

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