[Reaction of atrazine with protein and its application to detection of protein]

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[Reaction of atrazine with protein and its application to detection of protein] Abstract Text:

Deng-rong Shi,Tao Zhang,Li-ping Ren,Jin-lian Yu,Chun-rong Zhang,Zhen-hong Rao,

The resonance light scattering (RLS) technique and UV-Vis absorption spectra were applied to the investigation of the interaction between atrazine and bovine serum albumin(BSA). Under acidic conditions, the formation of atrazine-BSA supermolecule by Van der Waals force and N/O--H...pi hydrogen bonds leads to a red shift of absorption band and strong RLS enhancement of atrazine. The characteristics and intensity of RLS were related to the pH, the concentration of atrazine, and temperature. Under the optimum conditions, the enhanced RLS intensities are in proportion to the concentration of BSA in the range of 0.05-100 microg x mL(-1). Based on the enhancement of the RLS, a simple and sensitive method for the determination of BSA was established. The detection limit (3sigma) is 12 ng x mL(-1). Synthesis samples were determined with satisfactory results.

[Reaction of atrazine with protein and its application to detection of protein] Publishing Authors By Initials

DR Shi,T Zhang,LP Ren,JL Yu,CR Zhang,ZH Rao,

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[Reaction of atrazine with protein and its application to detection of protein] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Guang pu xue yu guang pu fen xi = Guang pu

VOLUME: 26

Page Numbers: 509-12

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ISSN: 1000-0593

DAY: 11

MONTH: Mar

YEAR: 2006

[Reaction of atrazine with protein and its application to detection of protein] Information

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LANGUAGE: chi

NlmUniqueID: 9424805

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Grant and Affiliation Information for [Reaction of atrazine with protein and its application to detection of protein]

AFFILIATION: Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100094, China.

Country: China

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MEDLINETA: Guang Pu Xue Yu Guang Pu Fen X

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