RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis.

RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Research Abstract Details 

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RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Abstract Text:

Christopher N Mayhew,Scott L Carter,Sejal R Fox,Charlene R Sexton,Christopher A Reed,Seetha V Srinivasan,Xiangdong Liu,Kathryn Wikenheiser-Brokamp,Gregory P Boivin,Ju-Seog Lee,Bruce J Aronow,Snorri S Thorgeirsson,Erik S Knudsen,

BACKGROUND & AIMS: The retinoblastoma (RB) tumor suppressor is functionally inactivated in most hepatocellular carcinomas (HCC), although the mechanisms by which RB suppresses liver tumorigenesis are poorly defined. We investigated the impact of RB loss on carcinogen-induced liver tumorigenesis. METHODS: Mice harboring liver-specific RB ablation and normal littermates were exposed to the hepatocarcinogen diethylnitrosamine (DEN). The influence of RB loss on liver tumorigenesis was assessed by evaluating tumor multiplicity, proliferation, and genome integrity within tumors arising in RB-deficient and wild-type livers. In silico analyses were used to probe the association between gene expression signatures for RB loss and chromosomal instability and the ability of genes up-regulated by RB loss to predict the survival of human HCC patients. RESULTS: RB deficiency significantly increased tumor multiplicity in livers exposed to DEN. Although hepatocytes in nontumor regions of DEN-exposed livers were quiescent regardless of RB status, tumors arising in RB-deficient livers were significantly more proliferative than those in normal livers and expressed high levels of RB/E2F target genes. Analysis of genes up-regulated by RB loss demonstrated significant overlap with a gene expression signature associated with chromosomal instability. Correspondingly, tumors arising in RB-deficient livers were significantly more likely to harbor hepatocytes exhibiting altered ploidy. Finally, gene expression analysis of human HCCs demonstrated that elevated expression of RB-regulated genes independently predicts poor survival. CONCLUSIONS: RB deletion in the mouse liver enhances DEN-induced tumorigenesis, associated with increased hepatocyte proliferation and compromised genome integrity. Evaluation of RB status may be a useful prognostic factor in human HCC.

RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Publishing Authors By Initials

CN Mayhew,SL Carter,SR Fox,CR Sexton,CA Reed,SV Srinivasan,X Liu,K Wikenheiser-Brokamp,GP Boivin,JS Lee,BJ Aronow,SS Thorgeirsson,ES Knudsen,

For similar proteins: dna-binding proteins: retinoblastoma protein research abstracts see: proteins: dna-binding proteins: retinoblastoma protein research

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RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Gastroenterology

VOLUME: 133

Page Numbers: 976-84

Journal Abbreviation: Gastroenterology

ISSN: 0016-5085

DAY: 20

MONTH: 06

YEAR: 2007

RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374630

RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Keywords Mesh Terms:

KEYWORDS: Retinoblastoma Protein

MESH TERMS: metabolism

Chemical & Substance for Abstract: RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis. Information

Substance Name: Diethylnitrosamine

Registry Number: 55-18-5

Grant and Affiliation Information for RB loss abrogates cell cycle control and genome integrity to promote liver tumorigenesis.

AFFILIATION: Department of Cell and Cancer Biology, University of Cincinnati, Cincinnati, Ohio 45267-0521, USA.

Country: United States

AGENCY: United States NIEHS

GRANT: U01 ES11038-02

ACRONYM: ES

MEDLINETA: Gastroenterology

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