Rational design and in vitro and in vivo delivery of Dicer substrate siRNA.

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Research Abstract Details 

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Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Abstract Text:

Mohammed Amarzguioui,Patric Lundberg,Edouard Cantin,James Hagstrom,Mark A Behlke,John J Rossi,Mohammed Amarzguioui,Patric Lundberg,Edouard Cantin,James Hagstrom,Mark A Behlke,John J Rossi,

RNA interference is a powerful tool for target-specific knockdown of gene expression. The triggers for this process are duplex small interfering RNAs (siRNAs) of 21-25 nt with 2-bp 3' overhangs produced in cells by the RNase III family member Dicer. We have observed that short RNAs that are long enough to serve as Dicer substrates (D-siRNA) can often evoke more potent RNA interference than the corresponding 21-nt siRNAs; this is probably a consequence of the physical handoff of the Dicer-produced siRNAs to the RNA-induced silencing complex. Here we describe the design parameters for D-siRNAs and a protocol for in vitro and in vivo intraperitoneal delivery of D-siRNAs and siRNAs to macrophages. siRNA delivery and transfection and analysis of macrophages in vivo can be accomplished within 36 h.

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Publishing Authors By Initials

M Amarzguioui,P Lundberg,E Cantin,J Hagstrom,MA Behlke,JJ Rossi,M Amarzguioui,P Lundberg,E Cantin,J Hagstrom,MA Behlke,JJ Rossi,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 1

Page Numbers: 508-17

Journal Abbreviation:

ISSN: 1750-2799

DAY: 3

MONTH: 12

YEAR: 2006

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Rational design and in vitro and in vivo delivery of Dicer substrate siRNA. Information

Substance Name: Ribonuclease III

Registry Number: EC 3.1.26.3

Grant and Affiliation Information for Rational design and in vitro and in vivo delivery of Dicer substrate siRNA.

AFFILIATION: The Biotechnology Centre of Oslo, Gaustadalleen 21, 0349 Oslo, Norway. moamarzg@biotek.uio.no

Country: England

AGENCY: United States NHLBI

GRANT: HL07470

ACRONYM: HL

MEDLINETA: Nat Protoc

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