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Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes.

Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Research Abstract Details 

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  • Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Abstract Text:

    k yamadaK Yamada,t sasakiT Sasaki,

    Rat liver cytosol fraction facilitated the transfer of [3H]glucosylceramide and [3H]-galactosylceramide from donor to acceptor liposomes, which contained one of these glycolipids in phospholipid-cholesterol. Lactosylceramide transfer activity was not detectable in the cytosol fraction. On Sephadex G-75 gel filtration of the liver cytosol fraction, both the glucosylceramide and galactosylceramide transfer activities were eluted at an identical position with a Kav value of 0.364, which corresponds to a molecular weight of 19,500; a little lactosylceramide transfer activity was found in the effluents containing the glycolipid transfer activities. The active protein fraction obtained by Sephadex G-75 chromatography of rat liver cytosol accelerated the transfer of galactosylceramide, glucosylceramide and lactosylceramide at relative rates of 100, 79, and 31, respectively. The rates of galactosylceramide and glucosylceramide transfer facilitated by the rat liver Sephadex G-75 fraction were not affected by either galactosylceramide, glucosylceramide, or lactosylceramide contained in the acceptor liposomes; acceptor liposomes lacking glycolipids acted efficiently as acceptors in the transfer reactions. For the lactosylceramide transfer reaction, significantly lower activity was detected when the acceptor liposomes contained either galactosylceramide or glucosylceramide. The translocation of [3H]-galactosylceramide from the donor to acceptor liposomes was confirmed by TLC and fluorography of the lipids extracted from the acceptor liposomes, which were separated from the donor liposomes after the incubation.

    Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Publishing Authors By Initials

    k yamadaK Yamada,t sasakiT Sasaki,

    For similar animals: animal population groups: animals, inbred strains: rats, inbred strains research abstracts see: animals: animal population groups: animals, inbred strains: rats, inbred strains research

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    Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 92

    Page Numbers: 457-64

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Aug

    YEAR: 1982

    Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Keywords Mesh Terms:

    KEYWORDS: Rats, Inbred Strains

    MESH TERMS: metabolism

    Chemical & Substance for Abstract: Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes. Information

    Substance Name: Cholesterol

    Registry Number: 57-88-5

    Grant and Affiliation Information for Rat liver glycolipid transfer protein. A protein which facilitates the translocation of mono- and dihexosylceramides from donor to acceptor liposomes.

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    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    ACRONYM:

    MEDLINETA: J Biochem

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