Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro.

Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Research Abstract Details 

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Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Abstract Text:

Daniel N Streblow,Koen W R van Cleef,Craig N Kreklywich,Christine Meyer,Patricia Smith,Victor Defilippis,Finn Grey,Klaus ,Robert Searles,Cathrien Bruggeman,Cornelis Vink,Jay A Nelson,Susan L Orloff,

Rat cytomegalovirus (RCMV) is a beta-herpesvirus with a 230-kbp genome containing over 167 open reading frames (ORFs). RCMV gene expression is tightly regulated in cultured cells, occurring in three distinct kinetic classes (immediate early, early, and late). However, the extent of viral-gene expression in vivo and its relationship to the in vitro expression are unknown. In this study, we used RCMV-specific DNA microarrays to investigate the viral transcriptional profiles in cultured, RCMV-infected endothelial cells, fibroblasts, and aortic smooth muscle cells and to compare these profiles to those found in tissues from RCMV-infected rat heart transplant recipients. In cultured cells, RCMV expresses approximately 95% of the known viral ORFs with few differences between cell types. By contrast, in vivo viral-gene expression in tissues from rat heart allograft recipients is highly restricted. In the tissues studied, a total of 80 viral genes expressing levels twice above background (5,000 to 10,000 copies per mug total RNA) were detected. In each tissue type, there were a number of genes expressed exclusively in that tissue. Although viral mRNA and genomic DNA levels were lower in the spleen than in submandibular glands, the number of individual viral genes expressed was higher in the spleen (60 versus 41). This finding suggests that the number of viral genes expressed is specific to a given tissue and is not dependent upon the viral load or viral mRNA levels. Our results demonstrate that the profiles, as well as the amplitude, of viral-gene expression are tissue specific and are dramatically different from those in infected cultured cells, indicating that RCMV gene expression in vitro does not reflect viral-gene expression in vivo.

Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Publishing Authors By Initials

DN Streblow,KW van Cleef,CN Kreklywich,C Meyer,P Smith,V Defilippis,F Grey,K ,R Searles,C Bruggeman,C Vink,JA Nelson,SL Orloff,

For similar surgical procedures, operative: transplantation: transplantation, homologous research abstracts see: surgical procedures, operative: transplantation: transplantation, homologous research

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Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 81

Page Numbers: 3816-26

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 24

MONTH: 01

YEAR: 2007

Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 113724

Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Keywords Mesh Terms:

KEYWORDS: Transplantation, Homologous

MESH TERMS: virology

Chemical & Substance for Abstract: Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro. Information

Substance Name: RNA, Viral

Registry Number: 0

Grant and Affiliation Information for Rat cytomegalovirus gene expression in cardiac allograft recipients is tissue specific and does not parallel the profiles detected in vitro.

AFFILIATION: Department of Molecular Microbiology and Immunology, The Vaccine and Gene Therapy Institute, Oregon Health and Science University, 505 SW 185th St., Beaverton, OR 97006, USA. streblow@ohsu.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL71695

ACRONYM: HL

MEDLINETA: J Virol

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