Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure.

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Abstract Text:

Yuangang Zu,Yujie Fu,Shuangming Li,Rui Sun,Qingyong Li, Schwarz,Yuangang Zu,Yujie Fu,Shuangming Li,Rui Sun,Qingyong Li, Schwarz,

A method is described for the simultaneous determination of paclitaxel and three related taxoids, 10-deacetylbaccatin III (10-DAB III), baccatin III, and cephalomannine, in the extracts from the needles of three Chinese yew species, Taxus cuspidata, T. chinensis, and T. media. SPE was applied as the sample preparation technique and RP-HPLC with a photodiode array detector (PAD) was used for the analysis of extract samples. The crude extracts were treated with an improved SPE cartridge packed with a combination of 1-vinyl-pyrrolidin-2-one and divinyl-benzene. The eluent was 75% methanol. The following separation was achieved with a gradient program on an HIQ SIL C18W column in a system of ACN/water within 60 min. The samples were detected by PAD at wavelengths of 232.1 nm for 10-DAB III, 229.8 nm for baccatin III and paclitaxel, and 223.9 nm for cephalomannine. The content of 10-DAB III, baccatin III, cephalomannine, and paclitaxel varied from 0.0277 to 0.0875, 0.0254 to 0.0405, 0.0715 to 0.2486, and 0.0996 to 0.1301 mg/g in fresh needles of the above yew species, respectively. The assay achieved good resolution in the separation between the four compounds, and it can be used for quality control or purity determination for those in bulk and pharmceutical dosage forms.

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Publishing Authors By Initials

Y Zu,Y Fu,S Li,R Sun,Q Li,G Schwarz,Y Zu,Y Fu,S Li,R Sun,Q Li,G Schwarz,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of separation science

VOLUME: 29

Page Numbers: 1237-44

Journal Abbreviation:

ISSN: 1615-9306

DAY: 12

MONTH: Jun

YEAR: 2006

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088554

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure.

AFFILIATION: Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, P. R. China.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Sep Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC PAD procedure Related Publications

• A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport.
• Activation of TLR2 and TLR4 by glycosylphosphatidylinositols derived from Toxoplasma gondii.
• Activity of alkaline phosphatase in uterine flushings of dairy cows affected with ovarian cysts or endometritis.
• Airway management and anesthesia in neonates, infants and children during endolaryngotracheal surgery.
• Aminoglycoside resistance in members of the Staphylococcus sciuri group.
• Biofuels from microbes.
• Complete nucleotide sequence of a small qnrS1-carrying plasmid from Salmonella enterica subsp. enterica Typhimurium DT193.
• Correlates of behavioral care management strategies used by primary care pediatric providers.
• Current treatment options in uterine endometrial stromal sarcoma: report of a case and review of the literature.
• Degradation of heterocyclic aromatic amines in oil under storage and frying conditions and reduction of their mutagenic potential.
• Dislocated epitaxial islands.
• Does current knowledge explain the pathogenesis of idiopathic pulmonary fibrosis? A perspective.
• Dopamine D2/D3 receptor stimulation fails to promote dopaminergic neurogenesis of murine and human midbrain-derived neural precursor cells in vitro.
• Erectile dysfunction: risk factor or manifestation of cardiovascular disease?
• Exploring the mental number line: evidence from a dual-task paradigm.
• Identification of flavonoids and hydroxycinnamic acids in pak choi varieties (Brassica campestris L. ssp. chinensis var. communis) by HPLC-ESI-MSn and NMR and their quantification by HPLC-DAD.
• Investigating the location of propyl gallate at surfaces and its chemical microenvironment by (1)H NMR.
• Management of hypertension complicated by psychiatric comorbidity.
• Menstrual irregularity from hormonal contraception: a cause of reproductive health concerns in minority adolescent young women.
• Molecular mechanisms of decreased susceptibility to fluoroquinolones in avian Salmonella serovars and their mutants selected during the determination of mutant prevention concentrations.
• Nondecoupling of maximal supergravity from the superstring.
• Preparation and execution of saccades: the problem of limited capacity of computational resources.
• Proteome analysis of chemically induced mouse liver tumors with different genotype.
• Rapid separation of four main taxoids in Taxus species by a combined LLP-SPE-HPLC (PAD) procedure.
• Recent advances in gene delivery for structural bone allografts.
• Recent developments in superstring theory.
• Serum components and activated Ha-ras antagonize expression of perivenous marker genes stimulated by beta-catenin signaling in mouse hepatocytes.
• The location of phenolic antioxidants and radicals at interfaces determines their activity.
• The nature of the stimulatory role of the supernatant fraction on triglyceride synthesis by the alpha-Glycerophosphate pathway.
• [In Process Citation]