Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry.

Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry. Research Abstract Details 

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Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry. Abstract Text:

Dandan Wang,Minli Zhang,Dandan Wang,Minli Zhang,

A rapid and sensitive ultra-performance liquid chromatography and mass spectrometry (UPLC/MS) method was developed to simultaneously quantify seven monohydroxyl testosterone metabolites (16alpha-, 2alpha-, 7alpha-, 6alpha-, 2beta-, 6beta-, and 16beta-hydroxyl testosterones) in rat liver microsomes. The UPLC system used a short 1.7-microm particle size column coupled to a Sciex 4000 Q trap in multiple reaction monitor (MRM) mode. All hydroxyl testosterones were resolved within 2.5 min. A 4-day validation was performed to determine the linearity, repeatability, reproducibility and accuracy of the method in rat liver microsomes. This method is applicable to the measurement of the testosterone hydroxylase activity in biological matrices such as the liver microsome incubates.

Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry. Publishing Authors By Initials

D Wang,M Zhang,D Wang,M Zhang,

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Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 855

Page Numbers: 290-4

Journal Abbreviation: J. Chromatogr. B Analyt. Techn

ISSN: 1570-0232

DAY: 29

MONTH: 05

YEAR: 2007

Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Rapid quantitation of testosterone hydroxyl metabolites by ultra-performance liquid chromatography and mass spectrometry.

AFFILIATION: Drug Metabolism and Pharmacokinetics, AstraZeneca Pharmaceuticals LP, Wilmington, DE 19850, USA. dandan.wang@astrazeneca.com

Country: Netherlands

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MEDLINETA: J Chromatogr B Analyt Technol

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