Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express.

Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Research Abstract Details 

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Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Abstract Text:

Xiuyun Wang,Runxiang Qiu,Walter Tsark,Qiang Lu,Xiuyun Wang,Runxiang Qiu,Walter Tsark,Qiang Lu,Xiuyun Wang,Runxiang Qiu,Walter Tsark,Qiang Lu,

Characterization of neural promoter/enhancers is essential for understanding gene regulation during brain development and provides useful genetic tools. However, it relies on the use of transgenic mice. We report a method for the rapid in vivo analysis of neural promoter/enhancers in the developing mouse brain and its application in the isolation of the doublecortin (DCX) promoter/enhancer for genetic labeling of young neurons. In the present study, we demonstrated that reporter genes introduced into the developing mouse cerebral cortex by in utero electroporation can achieve promoter/enhancer-specific patterns of expression. We used the in utero electroporation system to isolate a genomic fragment of the doublecortin gene that can direct reporter expression faithful to doublecortin in young neurons of the cerebral cortex. Finally, we showed that the DCX promoter identified via electroporation could reproduce doublecortin expression in the entire central nervous system in DCX-DsRed-express transgenic mice. The results of our study provide a convenient, reliable, and rapid method for in vivo analysis of neural promoter/enhancers in the developing mouse brain.

Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Publishing Authors By Initials

X Wang,R Qiu,W Tsark,Q Lu,X Wang,R Qiu,W Tsark,Q Lu,X Wang,R Qiu,W Tsark,Q Lu,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neuroscience research

VOLUME: 85

Page Numbers: 3567-73

Journal Abbreviation: J. Neurosci. Res.

ISSN: 0360-4012

DAY: 19

MONTH: Dec

YEAR: 2007

Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Information

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LANGUAGE: eng

NlmUniqueID: 7600111

Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: methods

Chemical & Substance for Abstract: Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express. Information

Substance Name: Cyclin-Dependent Kinase 5

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Rapid promoter analysis in developing mouse brain and genetic labeling of young neurons by doublecortin-DsRed-express.

AFFILIATION: Division of Neurosciences, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 91010, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS 052388

ACRONYM: NS

MEDLINETA: J Neurosci Res

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