[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot]

[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot] Research Abstract Details 

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[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot] Abstract Text:

Li-yan Li,Qiu-hua Mo,Xiang-min Xu,

OBJECTIVE: To establish a rapid and convenient method of reverse dot blot (RDB) analysis for detecting the point mutations of non-deletion alpha-thalassemia in Chinese. METHODS: Label biotin to primers and amplify human alpha2 globin gene selectively, then hybridize PCR products with a set of oligonucleotide probes immobilized on strips, and develop colour to detect non-deletion alpha-thalassemia defects. RESULTS: The PCR system using biotin-labeled primers could specifically amplify a 1085 bp fragment of human alpha2 globin gene which encompasses all six alpha-thalassemia mutations. After being hybridized with sequence-specific oligonucleotide probes and colour development, it could simultaneously identify all six types of non-deletion alpha-thalassemias encountered in Chinese. CONCLUSION: This method does not need semi-nested PCR, and the products amplified by biotinylated primers can be used directly to hybridize with the probes on strips under the identical conditions of hybridization. So, it is a specific and multiplex detection assay for screening non-deletion alpha-thalassemia defects in Chinese.

[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot] Publishing Authors By Initials

LY Li,QH Mo,XM Xu,

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[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yix

VOLUME: 20

Page Numbers: 345-7

Journal Abbreviation: Zhonghua Yi Xue Yi Chuan Xue Z

ISSN: 1003-9406

DAY: 6

MONTH: Aug

YEAR: 2003

[Rapid diagnosis of non-deletion alpha-thalassemias by reverse dot blot] Information

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LANGUAGE: chi

NlmUniqueID: 9425197

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AFFILIATION: Department of Medical Genetics, First Military Medical University, Guangzhou, Guangdong, 510515 PR China.

Country: China

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MEDLINETA: Zhonghua Yi Xue Yi Chuan Xue Z

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