Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections.

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Abstract Text:

K Belák,K Funa,R Kelly,S Belák,

A practical in situ hybridization (ISH) method has been developed for the rapid diagnosis of pseudorabies virus (PRV) infection in pigs. The method utilizes routine formalin fixed and paraffin-embedded tissue sections, non radioactive biotin-labelling, simple hybridization procedure and diaminobenzidine (DAB) silver enhancement detection system. The rapid (ISH) method showed concordance with the direct filter hybridization (DFH) and with the peroxidase-antiperoxidase (PAP) assays. By the simplified ISH, diagnosis can be made within two days.

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Publishing Authors By Initials

K Belák,K Funa,R Kelly,S Belák,

For similar animal diseases: swine diseases research abstracts see: animal diseases: swine diseases research

PUBMED ID PMID:

MEDLINE DATE:

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 36

Page Numbers: 10-20

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Feb

YEAR: 1989

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 331325

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Keywords Mesh Terms:

KEYWORDS: Swine Diseases

MESH TERMS: diagnosis

Chemical & Substance for Abstract: Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections.

AFFILIATION:

Country: GERMANY, WEST

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zentralbl Veterinarmed B

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections Related Publications

• Analysis of ORFs 2b, 3, 4, and partial ORF5 of sequential isolates of equine arteritis virus shows genetic variation following experimental infection of horses.
• Characterization of the glycoprotein B gene from ruminant alphaherpesviruses.
• Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations.
• Detection of bovine adenovirus nucleic acid sequences in nasal specimens by biotinylated DNA probes.
• Development of a real-time PCR assay based on primer-probe energy transfer for the detection of swine vesicular disease virus.
• Direct detection of bovine leukemia virus infection: practical applicability of a double polymerase chain reaction.
• Experiences of an OIE Collaborating Centre in molecular diagnosis of transboundary animal diseases: a review.
• Experimental infection of weaned calves and lambs with two strains of bovine adenovirus type 2.
• Genomic and pathogenic studies on a glycoprotein E variant field isolate of bovine herpesvirus 1.
• Highly diverse type of equine arteritis virus (EAV) from the semen of a South African donkey: short communication.
• Identification and sequence analysis of the glycoprotein B gene of porcine cytomegalovirus.
• Improved diagnosis for nine viral diseases considered as notifiable by the world organization for animal health.
• Rapid diagnosis of Aujeszky's disease in pigs by improved in situ hybridization using biotinylated probes on paraffin-embedded tissue sections.
• Simultaneous genotyping of all hemagglutinin and neuraminidase subtypes of avian influenza viruses by use of padlock probes.