Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus.

Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus. Research Abstract Details 

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Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus. Abstract Text:

Keita Yamada,Satomi Hyodo,Yu-ki Matsuno,Mitsuhiro Kinoshita,Shu-zou Maruyama,Yu-suke Osaka,Enriqueta Casal,Yuan C Lee,Kazuaki Kakehi,Keita Yamada,Satomi Hyodo,Yu-ki Matsuno,Mitsuhiro Kinoshita,Shu-zou Maruyama,Yu-suke Osaka,Enriqueta Casal,Yuan C Lee,Kazuaki Kakehi,

Rapid and sensitive analysis of glycans is essential for glycomics. We previously reported an apparatus, the AutoGlycoCutter (AGC), for rapid release of O-linked glycans under alkaline conditions and its application to rapid analysis of glycans in proteoglycans. We now report an application of the AGC to obtain mucin-type glycans with reducing end (i.e., hemiacetal group) within only 3 min. The released oligosaccharides could be labeled with fluorescent 2-aminobenzoic acid for analysis by normal-phase high-performance liquid chromatography (NP-HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). We could detect O-glycans from as low as 5 pmol of bovine caseino glycomacropeptide (CGMP) by the proposed procedures. The validity of the current method was shown by the analyses of the released O-glycans from some standard glycoproteins: bovine submaxillary mucin, bovine fetuin, porcine stomach mucin, and human colostrum immunoglobulin A. The advantage of the current method was also demonstrated in comparative analysis of mucin-type glycans in CGMP derived from three different animal species.

Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus. Publishing Authors By Initials

K Yamada,S Hyodo,YK Matsuno,M Kinoshita,SZ Maruyama,YS Osaka,E Casal,YC Lee,K Kakehi,K Yamada,S Hyodo,YK Matsuno,M Kinoshita,SZ Maruyama,YS Osaka,E Casal,YC Lee,K Kakehi,

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Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical biochemistry

VOLUME: 371

Page Numbers: 52-61

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 15

MONTH: 06

YEAR: 2007

Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Rapid and sensitive analysis of mucin-type glycans using an in-line flow glycan-releasing apparatus.

AFFILIATION: Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Osaka 577-8502, Japan.

Country: United States

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MEDLINETA: Anal Biochem

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