A multi-wall carbon nanotubes (MWNTs)/Nafion modified glassy carbon electrode (GCE) was fabricated for the rapid amperometric detection of coliforms, represented by Escherichia coli (E. coli). In the bacterial solution, beta-galactosidase which was used as an indicator of coliforms reacted with substrate, p-aminophenol-beta-galactopyranoside (PAPG), and produced p-aminophenol (PAP). PAP was detected by MWNTs/Nafion modified GCE. Due to the cation-exchange capacity of Nafion and the electrocatalytic ability of MWNTs, the detection sensitivity of PAP was improved and the detection time of coliforms was shortened. The bacterial can be detected within 5h ranging from 10 to 10(4)cfu/mL. The MWNTs/Nafion modified GCE was easy to be constructed and regenerated. To our best knowledge, it was the first time to use MWNTs/Nafion modified GCE to detect the concentration of coliforms.
Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode. Publishing Authors By Initials
Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode. Journal Published:
PUBLICATION TYPE: Research Support, Non-U.S. Gov
Journal: Talanta
VOLUME: 75
Page Numbers: 167-71
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ISSN: 1873-3573
DAY: 9
MONTH: 11
YEAR: 2007
Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode. Information
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LANGUAGE: eng
NlmUniqueID: 2984816
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Grant and Affiliation Information for Rapid amperometric detection of coliforms based on MWNTs/Nafion composite film modified glass carbon electrode.
AFFILIATION: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
Country: England
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MEDLINETA: Talanta
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