Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors.

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Abstract Text:

Robert M Lober,Miguel A Pereira,Nevin A Lambert,

G-protein-coupled receptors (GPCRs) mediate slow synaptic transmission and many other effects of small molecule and peptide neurotransmitters. In the standard model of GPCR signaling, receptors and G-proteins diffuse laterally within the plane of the plasma membrane and encounter each other by random collision. This model predicts that signaling will be most efficient if both GPCRs and G-proteins are free to diffuse, thus maximizing collision frequency. However, neuronal GPCRs are often recruited to and enriched at specific synaptic locations, suggesting receptor mobility is restricted in these cells. Here, we test the hypothesis that restricting GPCR mobility impairs signaling in neurons by limiting the frequency of collisions between receptors and G-proteins. Mu-opioid receptors (MORs) were immobilized on the surface of cerebellar granule neurons by avidin-mediated cross-linking, and inwardly rectifying potassium (GIRK) channels were used as rapid indicators of G-protein activation. Mobile and immobile MORs activated GIRK channels with the same onset kinetics and agonist sensitivity in these neurons. In a heterologous expression system, GFP (green fluorescent protein)-tagged G alpha(oA) subunits remained mobile after cross-linking, but their mobility was reduced in the presence of immobile MORs, suggesting that these receptors and subunits were transiently precoupled. In addition, channel activation could be reconstituted with immobile GPCRs, G-protein heterotrimers, and GIRK channels. These results show that collision frequency is not rate-limiting for G-protein activation in CNS neurons, and are consistent with the idea that signaling components are compartmentalized or preassembled.

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Publishing Authors By Initials

RM Lober,MA Pereira,NA Lambert,

For similar synaptic transmission research abstracts see: synaptic transmission research

PUBMED ID PMID:

MEDLINE DATE:

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of neuroscience : the official journal

VOLUME: 26

Page Numbers: 12602-8

Journal Abbreviation: J. Neurosci.

ISSN: 1529-2401

DAY: 29

MONTH: Nov

YEAR: 2006

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8102140

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Keywords Mesh Terms:

KEYWORDS: Synaptic Transmission

MESH TERMS: physiology

Chemical & Substance for Abstract: Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors. Information

Substance Name: Receptors, G-Protein-Coupled

Registry Number: 0

Grant and Affiliation Information for Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors.

AFFILIATION: Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta, Georgia 30809, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS36455

ACRONYM: NS

MEDLINETA: J Neurosci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors Related Publications

• Active Galpha(q) subunits and M3 acetylcholine receptors promote distinct modes of association of RGS2 with the plasma membrane.
• Androgen metabolites in bovine follicular fluid.
• Anxiety, depression and coping strategies in post-hysterectomy Chinese women prior to discharge.
• Asymmetric ERM activation at the Schwann cell process tip is required in axon-associated motility.
• Changes in heart rate recovery in response to acute changes in training load.
• Critical roles for non-pRb targets of human papillomavirus type 16 E7 in cervical carcinogenesis.
• Domains in the XPA protein important in its role as a processivity factor.
• Factors associated with menstrual dysfunction and self-reported bone stress injuries in female runners in the ultra- and half-marathons of the Two Oceans.
• Gain is not always good.
• HPV16 E6 confers p53-dependent and p53-independent phenotypes in the epidermis of mice deficient for E6AP.
• Human papillomavirus 16 E5 oncogene contributes to two stages of skin carcinogenesis.
• Identification of biomarkers that distinguish human papillomavirus (HPV)-positive versus HPV-negative head and neck cancers in a mouse model.
• In vitro pharmacological characterisation of a novel cyclic nociceptin/orphanin FQ analogue c[Cys(7,10)]N/OFQ(1-13)NH (2).
• Induction of promyelocytic leukemia (PML) oncogenic domains (PODs) by papillomavirus.
• Infectious disease among enslaved African Americans at Eaton's Estate, Warren County, North Carolina, ca. 1830-1850.
• Metabolic consequences of exercise-induced muscle damage.
• Molecular evolution of hemojuvelin and the repulsive guidance molecule family.
• Multiple sites of exposure in an outbreak of ornithosis in workers at a poultry abattoir and farm.
• Neurosteroid modulation of synaptic and extrasynaptic GABA(A) receptors.
• Prevalence and clinical phenotype of the p.Val226Met glucokinase gene mutation in French Canadians in Quebec, Canada.
• Rapid activation of inwardly rectifying potassium channels by immobile G-protein-coupled receptors.
• Some G protein heterotrimers physically dissociate in living cells.
• Sympathetic activity in major depressive disorder: identifying those at increased cardiac risk?
• The Effect Of Fat-adaptation Followed By Carbohydrate-loading On Ultra-endurance Cycling Performance: 803: June 1 8:15 AM - 8:30 AM.
• The effect of bone marrow transplants on DNA profiles; a case example.
• The neuronal noradrenaline transporter, anxiety and cardiovascular disease.
• Vitamin D deficiency.
• Workshop of experts: definition, recognition, assessment, and alleviation of animal distress in the laboratory.
• [In Process Citation]
• alphaII-Spectrin interacts with five groups of functionally important proteins in the nucleus.