RAPD markers linked to eastern filbert blight resistance in Corylus avellana.

RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Research Abstract Details 

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RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Abstract Text:

S A Mehlenbacher,R N Brown,J W Davis,H Chen,N V Bassil,D C Smith,T L Kubisiak,

A total of 1,110 decamer primers were screened for RAPD markers linked to a dominant allele in hazelnut ( Corylus avellana) that confers resistance to eastern filbert blight caused by Anisogramma anomala. Twenty RAPD markers linked in coupling, and five markers linked in repulsion, were found. A seedling population was used to construct a linkage map of the region flanking the resistance locus. The map spans 46.6 cM, with 14 markers on one side of the resistance locus and eight on the other side. Eleven markers showed less than 3% recombination with resistance, including three that showed no recombination. Seven of these 11 markers are sufficiently robust to allow their use in marker-assisted selection. These include AA12(850) which shows no recombination, and six markers on one side of the resistance locus: 173(500), 152(800), 122(825), 275(1130), H19(650) and O16(1250). Marker 268(580), which flanks the resistance locus on the other side, is also suitable for use in marker-assisted selection, but shows 5.8% recombination with resistance. Other markers are less suitable for marker-assisted selection because of sensitivity to changes in primer or MgCl(2) concentration, or the long time required for electrophoresis to separate bands of similar size. The 16 markers closest to the resistance locus were cloned and sequenced. The W07(365) marker, which showed no recombination with the resistance locus but is difficult to score, includes a CT microsatellite repeat. The sequence information will allow the design of SCAR primers and eventual map-based cloning of the resistance allele.

RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Publishing Authors By Initials

SA Mehlenbacher,RN Brown,JW Davis,H Chen,NV Bassil,DC Smith,TL Kubisiak,

For similar investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research abstracts see: investigative techniques: genetic techniques: sequence analysis: sequence analysis, dna research

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RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 108

Page Numbers: 651-6

Journal Abbreviation:

ISSN: 0040-5752

DAY: 21

MONTH: 10

YEAR: 2003

RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Information

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LANGUAGE: eng

NlmUniqueID: 145600

RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Keywords Mesh Terms:

KEYWORDS: Sequence Analysis, DNA

MESH TERMS: microbiology

Chemical & Substance for Abstract: RAPD markers linked to eastern filbert blight resistance in Corylus avellana. Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for RAPD markers linked to eastern filbert blight resistance in Corylus avellana.

AFFILIATION: Department of Horticulture, Oregon State University, 4017 ALS Building, Corvallis, OR 97331, USA. mehlenbs@science.oregonstate.edu

Country: Germany

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MEDLINETA: Theor Appl Genet

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