Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae.

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Abstract Text:

Iben Plate,Line Albertsen,Michael Lisby,Swee C L Hallwyl,Qi Feng,Changhyun Seong,Rodney Rothstein,Patrick Sung,Uffe H Mortensen,Iben Plate,Line Albertsen,Michael Lisby,Swee C L Hallwyl,Qi Feng,Changhyun Seong,Rodney Rothstein,Patrick Sung,Uffe H Mortensen,

Rad52 is essential for all homologous recombination and DNA double strand break repair events in Saccharomyces cerevisiae. This protein is multifunctional and contains several domains that allow it to interact with DNA as well as with different repair proteins. However, it has been unclear how Rad52 enters the nucleus. In the present study, we have used a combination of mutagenesis and sequence analysis to show that Rad52 from S. cerevisiae contains a single functional pat7 type NLS essential for its nuclear localization. The region containing the NLS seems only to be involved in nuclear transport as it plays no role in repair of MMS-induced DNA damage. The NLS in Rad52 is weak, as monomeric protein species that harbor this NLS are mainly located in the cytosol. In contrast, multimeric protein complexes wherein each subunit contains a single NLS(Rad52) sort efficiently to the nucleus. Based on the results we propose a model where the additive effect of multiple NLS(Rad52) sequences in a Rad52 ring-structure ensures efficient nuclear localization of Rad52.

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Publishing Authors By Initials

I Plate,L Albertsen,M Lisby,SC Hallwyl,Q Feng,C Seong,R Rothstein,P Sung,UH Mortensen,I Plate,L Albertsen,M Lisby,SC Hallwyl,Q Feng,C Seong,R Rothstein,P Sung,UH Mortensen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: DNA repair

VOLUME: 7

Page Numbers: 57-66

Journal Abbreviation: DNA Repair (Amst.)

ISSN: 1568-7864

DAY: 20

MONTH: 09

YEAR: 2007

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101139138

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae.

AFFILIATION: Center for Microbial Biotechnology, BioCentrum-DTU, Technical University of Denmark, Bldg. 223, DK-2800 Kgs. Lyngby, Denmark.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: DNA Repair (Amst)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae Related Publications

• Bladder-neck obstruction; a review of 508 cases treated by endoscopic resection.
• Bodyweight changes associated with antihyperglycaemic agents in type 2 diabetes mellitus.
• Choledocho-choledochostomy: the natural history of healing in pigs.
• Comment on "pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel".
• Complete sagittal septum of the bladder. A case report.
• Direct monitoring pressure overload predicts cardiac hypertrophy in mice.
• Diverticulum of the gastric cardia.
• Epidemiology of autoimmune diseases in Denmark.
• Esophageal cancer: biology, natural history, staging and therapeutic options.
• Family and partner psychopathology and the risk of postpartum mental disorders.
• Go but not Gi2 or Gi3 is required for muscarinic regulation of heart rate and heart rate variability in mice.
• Hitting bacteria at the heart of the central dogma: sequence-specific inhibition.
• Hypotension, lipodystrophy, and insulin resistance in generalized PPARgamma-deficient mice rescued from embryonic lethality.
• Indicators of fetal growth and bipolar disorder: a Danish national register-based study.
• Is botulinum toxin injection a better treatment than nitroglycerin ointment for patients with chronic anal fissure?
• Lost life years attributable to stroke among patients with nonvalvular atrial fibrillation: a nationwide population-based follow-up study.
• Obstetric conditions and risk of first admission with schizophrenia: A Danish national register based study.
• PPAR-gamma knockout in pancreatic epithelial cells abolishes the inhibitory effect of rosiglitazone on caerulein-induced acute pancreatitis.
• Pharmacokinetic characterization of a pig model of ciclosporin A nephrotoxicity following intravenous administration.
• Production and epitope characterization of mAbs specific for translation factor IF1.
• Rad52 multimerization is important for its nuclear localization in Saccharomyces cerevisiae.
• Radiofrequency ablation of colorectal liver metastases: long-term survival?
• Surgical therapy of perianal Crohn's disease.
• The impact of nonlinear exposure-risk relationships on seasonal time-series data: modelling Danish neonatal birth anthropometric data.
• The trend in urological practice abroad.
• Transient marker system for iterative gene targeting of a prototrophic fungus.
• Transplacental transfer of monomethyl phthalate and mono(2-ethylhexyl) phthalate in a human placenta perfusion system.
• Transthoracic nephrectomy.
• [Formoguanamine as a diuretic.]
• [In Process Citation]