RACK1 is a BKCa channel binding protein.

RACK1 is a BKCa channel binding protein. Research Abstract Details 

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RACK1 is a BKCa channel binding protein. Abstract Text:

Christina Kaldany Isacson,Qing Lu,Richard H Karas,Daniel H Cox,

The large conductance calcium-activated potassium channel, or BK(Ca) channel, plays an important feedback role in a variety of physiological processes, including neurotransmitter release and smooth muscle contraction. Some reports have suggested that this channel forms a stable complex with regulators of its function, including several kinases and phosphatases. To further define such signaling complexes, we used the yeast two-hybrid system to screen a human aorta cDNA library for proteins that bind to the BK(Ca) channel's intracellular, COOH-terminal "tail". One of the interactors we identified is the protein receptor for activated C kinase 1 (RACK1). RACK1 is a member of the WD40 protein family, which also includes the G protein beta-subunits. Consistent with an important role in BK(Ca)-channel regulation, RACK1 has been shown to be a scaffolding protein that interacts with a wide variety of signaling molecules, including cSRC and PKC. We have confirmed the interaction between RACK1 and the BK(Ca) channel biochemically with GST pull-down and coimmunoprecipitation experiments. We have observed some co-localization of RACK1 with the BK(Ca) channel in vascular smooth muscle cells with immunocytochemical experiments, and we have found that RACK1 has effects on the BK(Ca) channel's biophysical properties. Thus RACK1 binds to the BK(Ca) channel and it may form part of a BK(Ca)-channel regulatory complex in vascular smooth muscle.

RACK1 is a BKCa channel binding protein. Publishing Authors By Initials

CK Isacson,Q Lu,RH Karas,DH Cox,

For similar animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research abstracts see: animals: chordata: vertebrates: amphibia: anura: pipidae: xenopus: xenopus laevis research

PUBMED ID PMID:

MEDLINE DATE:

RACK1 is a BKCa channel binding protein. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Cell physiology

VOLUME: 292

Page Numbers: C1459-66

Journal Abbreviation:

ISSN: 0363-6143

DAY: 13

MONTH: 12

YEAR: 2006

RACK1 is a BKCa channel binding protein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901225

RACK1 is a BKCa channel binding protein. Keywords Mesh Terms:

KEYWORDS: Xenopus laevis

MESH TERMS: physiology

Chemical & Substance for Abstract: RACK1 is a BKCa channel binding protein. Information

Substance Name: GTP-Binding Proteins

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for RACK1 is a BKCa channel binding protein.

AFFILIATION: Molecular Cardiology Research Institute, Tufts-New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01-HL-64831

ACRONYM: HL

MEDLINETA: Am J Physiol Cell Physiol

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