Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line.

Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Research Abstract Details 

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Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Abstract Text:

Hiroyo Oda,Harumi Suzuki,Kouhei Sakai,Seiji Kitahara,Michael S Patrick,Yoshinao Azuma,Kazuro Sugi,Toshio Kitamura,Jonathan Kaye,Mutsunori Shirai,

Rac1, one of the Rho family small guanosine triphosphatases, has been shown to work as a "molecular switch" in various signal transduction pathways. To assess the function of Rac1 in the differentiation process of CD4 single-positive (CD4-SP) T cells from CD4CD8 double-positive (DP) cells, we used a DP cell line DPK, which can differentiate into CD4-SP cells upon TCR stimulation in vitro. DPK expressing dominant-negative (dn)Rac1 underwent massive apoptosis upon TCR stimulation and resulted in defective differentiation of CD4-SP cells. Conversely, overexpression of dnRac2 did not affect differentiation. TCR-dependent actin polymerization was inhibited, whereas early ERK activation was unaltered in dnRac1-expressing DPK. We found that TCR-dependent induction of Bcl-2 was suppressed greatly in dnRac1-expressing DPK, and this suppression was independent of actin rearrangement. Furthermore, introduction of exogenous Bcl-2 inhibited TCR-dependent induction of apoptosis and restored CD4-SP generation in dnRac1-expressing DPK without restoring TCR-induced actin polymerization. Collectively, these data indicate that Rac1 is critical in differentiation of CD4-SP from the DP cell line by preventing TCR-induced apoptosis via Bcl-2 up-regulation.

Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Publishing Authors By Initials

H Oda,H Suzuki,K Sakai,S Kitahara,MS Patrick,Y Azuma,K Sugi,T Kitamura,J Kaye,M Shirai,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins: rac gtp-binding proteins: rac1 gtp-binding protein research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: gtp phosphohydrolases: gtp-binding proteins: monomeric gtp-binding proteins: rho gtp-binding proteins: rac gtp-binding proteins: rac1 gtp-binding protein research

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Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of leukocyte biology

VOLUME: 81

Page Numbers: 500-8

Journal Abbreviation: J. Leukoc. Biol.

ISSN: 0741-5400

DAY: 7

MONTH: 11

YEAR: 2006

Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Information

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LANGUAGE: eng

NlmUniqueID: 8405628

Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Keywords Mesh Terms:

KEYWORDS: rac1 GTP-Binding Protein

MESH TERMS: metabolism

Chemical & Substance for Abstract: Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line. Information

Substance Name: rac1 GTP-Binding Protein

Registry Number: EC 3.6.5.2

Grant and Affiliation Information for Rac1-mediated Bcl-2 induction is critical in antigen-induced CD4 single-positive differentiation of a CD4+CD8+ immature thymocyte line.

AFFILIATION: Department of Microbiology and Immunology, Yamaguchi University School of Medicine, Ube, Japan.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI031231-14

ACRONYM: AI

MEDLINETA: J Leukoc Biol

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