Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX.

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Abstract Text:

Tuan Lin Tan,Ning Fang,Tuan Ling Neo,Pritpal Singh,Jianhua Zhang,Ruijie Zhou,Cheng-Gee Koh,Vincent Chan,Seng Gee Lim,Wei Ning Chen,Tuan Lin Tan,Ning Fang,Tuan Ling Neo,Pritpal Singh,Jianhua Zhang,Ruijie Zhou,Cheng-Gee Koh,Vincent Chan,Seng Gee Lim,Wei Ning Chen,

Hepatitis B virus (HBV) is a causative agent for liver diseases including hepatocellular carcinoma. Understanding its interactions with cellular proteins is critical in the elucidation of the mechanisms of disease progression. Using a cell-based HBV replication system, we showed that HBV replication in HepG2 cells resulted in a cellular morphological changes displaying membrane rufflings and lamellipodia like structures reminiscent of cells expressing constitutively activated Rac1. We also showed that activated Rac1 resulted in increased viral replication. HBV replication specifically activated wild type Rac1, but not Cdc42. The Rac1 activation by HBV replication also resulted in the phosphorylation of ERK1/2 and AKT, the downstream targets of Rac1 signaling cascade. The smallest HBV viral protein, HBX, was able to activate the endogenous Rac1 and induce membrane ruffling when transfected into cells. Significantly, HBX was found to directly interact with a Rac1 nucleotide exchange factor (betaPIX) through a SH3 binding motif. Taken together, we have shown the interaction of HBV with the Rho GTPase, affecting cell morphology through the Rac1 activation pathway. HBV may possibly make use of an activated Rac1 signaling pathway for increased replication and resultant metastatic effects.

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Publishing Authors By Initials

TL Tan,N Fang,TL Neo,P Singh,J Zhang,R Zhou,CG Koh,V Chan,SG Lim,WN Chen,TL Tan,N Fang,TL Neo,P Singh,J Zhang,R Zhou,CG Koh,V Chan,SG Lim,WN Chen,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochimica et biophysica acta

VOLUME: 1783

Page Numbers: 360-74

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 22

MONTH: 11

YEAR: 2007

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX.

AFFILIATION: School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459 Singapore; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, 637551 Singapore.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochim Biophys Acta

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Rac1 GTPase is activated by hepatitis B virus replication - involvement of HBX Related Publications

• A new function of BMP4: dual role for BMP4 in regulation of Sonic hedgehog expression in the mouse tooth germ.
• An experimental study of anti-angiogenesis with recombinant human kringle 5.
• Application of Nd-Yag laser for gingivoplasty during orthodontic treatment.
• Clinical study of alveolar vertical distraction osteogenesis for implant.
• Enzyme-linked immunosorbent assay for the determination of p21-activated kinase activity.
• Hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis.
• Lack of effect of dietary alpha-tocopherol on chemically induced hepatocarcinogenesis in rats.
• Prostaglandin E2 is involved in the increase of cytochrome P-450 2B1 expression by alpha-tocopheryl succinate in primary rat hepatocytes in the presence of phenobarbital.
• Rescue of cleft palate in Msx1-deficient mice by transgenic Bmp4 reveals a network of BMP and Shh signaling in the regulation of mammalian palatogenesis.
• Transgenically ectopic expression of Bmp4 to the Msx1 mutant dental mesenchyme restores downstream gene expression but represses Shh and Bmp2 in the enamel knot of wild type tooth germ.
• [A study on apoptosis and activity of SAPK/JNK in adrenocortical cells induced by cadmium chloride]
• [A study on central zone contour of tooth-crown vesticular surface among young people with normal occlusion]
• [Bone-inducing activity of human bone morphogenetic protein-2 102 peptide]
• [Comparative effects of tensiomin and sodium dimercaptosulphonate on the serum sulfydryl and anticopper of the patients with Wilson's disease]
• [Effects of fluoride on the expression of c-fos and c-jun genes and cell proliferation of rat osteoblasts]
• [Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping]
• [Experimental methods for mechanically stimulating the cells in vitro]
• [Focal adhesion kinase]
• [GATA-2 gene expression in leukemia patients and its significance]
• [Genetic polymorphisms of cytochrome P450 1A1 and susceptibility of early-onset Parkinson's disease]
• [Influence of chemotherapy on hematopoietic microenvironment and effect of autologous bone marrow stromal cell infusion on the recovery of hematopoiesis after chemotherapy]
• [Influence of the stratum corneum and dermis of the skin on the percutaneous absorption of fluorouracil delivered through skin patch]
• [Isolation, cultivation and identification of neural stem cell from human embryonic CNS]
• [Melanoma antigen-3 expression in human hepatocellular carcinoma]
• [Novel full-length cDNA cloning from normal adrenal gland and pheochromocytoma and functional prediction]
• [Preparation and application of a new chloromethylstyrene encapsulated silica single anion column]
• [Study on the intestinal microbial colonization resistance in patients with chronic severe hepatitis]
• [Synergetic transactivating functions of HCV core and truncated HBV middle surface protein]
• [Telomerase activity and cellular apoptosis of bladder cancers]
• [The apolipoprotein E gene polymorphism in children with steroid-resistant idiopathic nephrotic syndrome]