Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation.

Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Research Abstract Details 

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Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Abstract Text:

Michael R Warnement,Shannon L Faley,John P Wikswo,Sandra J Rosenthal,

Antibody-conjugated quantum dots (QDs) have been used to map the expression dynamics of the cytokine receptor interleukin-2 receptor-alpha (IL-2Ralpha) following Jurkat T cell activation. Maximal receptor expression was observed 48 h after activation, followed by a sharp decrease consistent with IL-2R internalization subsequent to IL-2 engagement. Verification of T cell activation and specificity of QD labeling were demonstrated using fluorescence microscopy, ELISA, and FACS analyses. These antibody conjugates provide a versatile means to rapidly determine cell state and interrogate membrane associated proteins involved in cell signaling pathways. Ultimately, incorporation with a microfluidic platform capable of simultaneously monitoring several cell signaling pathways will aid in toxin detection and discrimination.

Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Publishing Authors By Initials

MR Warnement,SL Faley,JP Wikswo,SJ Rosenthal,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research

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Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: IEEE transactions on nanobioscience

VOLUME: 5

Page Numbers: 268-72

Journal Abbreviation:

ISSN: 1536-1241

DAY: 3

MONTH: Dec

YEAR: 2006

Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101152869

Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: immunology

Chemical & Substance for Abstract: Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation. Information

Substance Name: Receptors, Cytokine

Registry Number: 0

Grant and Affiliation Information for Quantum dot probes for monitoring dynamic cellular response: reporters of T cell activation.

AFFILIATION: Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD15052

ACRONYM: HD

MEDLINETA: IEEE Trans Nanobioscience

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