Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors.

Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Research Abstract Details 

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Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Abstract Text:

Andrew C Paoletti,Tari J Parmely,Chieri Tomomori-Sato,Shigeo Sato,Dongxiao Zhu,Ronald C Conaway,Joan Weliky Conaway,Laurence Florens,Michael P Washburn,

Components of multiprotein complexes are routinely determined by using proteomic approaches. However, this information lacks functional content except when new complex members are identified. To analyze quantitatively the abundance of proteins in human Mediator we used normalized spectral abundance factors generated from shotgun proteomics data sets. With this approach we define a common core of mammalian Mediator subunits shared by alternative forms that variably associate with the kinase module and RNA polymerase (pol) II. Although each version of affinity-purified Mediator contained some kinase module and RNA pol II, Mediator purified through F-Med26 contained the most RNA pol II and the least kinase module as demonstrated by the normalized spectral abundance factor approach. The distinct forms of Mediator were functionally characterized by using a transcriptional activity assay, where F-Med26 Mediator/RNA pol II was the most active. This method of protein complex visualization has important implications for the analysis of multiprotein complexes and assembly of protein interaction networks.

Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Publishing Authors By Initials

AC Paoletti,TJ Parmely,C Tomomori-Sato,S Sato,D Zhu,RC Conaway,JW Conaway,L Florens,MP Washburn,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: nucleotidyltransferases: rna nucleotidyltransferases: dna-directed rna polymerases: rna polymerase ii research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: nucleotidyltransferases: rna nucleotidyltransferases: dna-directed rna polymerases: rna polymerase ii research

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Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 18928-33

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 30

MONTH: 11

YEAR: 2006

Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Keywords Mesh Terms:

KEYWORDS: RNA Polymerase II

MESH TERMS: metabolism

Chemical & Substance for Abstract: Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors. Information

Substance Name: RNA Polymerase II

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors.

AFFILIATION: Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO 64110, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R37 GM 41628

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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