Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages.

Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Research Abstract Details 

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Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Abstract Text:

Justin I Odegaard,Divya Vats,Lina Zhang,Roberto Ricardo-Gonzalez,Kristi L Smith,David B Sykes,Mark P Kamps,Ajay Chawla,

Macrophages participate in physiologic and pathologic processes through elaboration of distinct activation programs. Studies with macrophage cell systems have revealed much concerning the importance of this pleiotropic cell; however, these studies are inherently limited by three factors: heterogeneity of the target cell population, poor capacity to elaborate various activation programs, and lack of a genetically tractable model system for loss- and gain-of-function studies. Although definitive, hematopoietic lineages can be isolated from embryonic stem (ES) cells, these isolation procedures are inefficient and time-consuming and require elaborate cell-sorting protocols. We therefore examined whether myeloid precursors, capable of differentiating into macrophages, could be conditionally expanded in vitro. Here, we report methods for selective isolation and immortalization of ES cell-derived myeloid precursors by estrogen-regulated HoxA9 protein. Using this new macrophage differentiation system, an unlimited number of custom-designed macrophages with defined functional characteristics can be generated from any targeted ES cell. In combination with knockout or small interfering RNA knockdown technologies, this macrophage differentiation system provides a powerful tool for high throughput analysis of regulatory mechanisms controlling macrophage activation in health and disease.

Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Publishing Authors By Initials

JI Odegaard,D Vats,L Zhang,R Ricardo-Gonzalez,KL Smith,DB Sykes,MP Kamps,A Chawla,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes: cd4-positive t-lymphocytes: t-lymphocytes, helper-inducer: th2 cells research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes: cd4-positive t-lymphocytes: t-lymphocytes, helper-inducer: th2 cells research

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Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of leukocyte biology

VOLUME: 81

Page Numbers: 711-9

Journal Abbreviation: J. Leukoc. Biol.

ISSN: 0741-5400

DAY: 8

MONTH: 12

YEAR: 2006

Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8405628

Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Keywords Mesh Terms:

KEYWORDS: Th2 Cells

MESH TERMS: immunology

Chemical & Substance for Abstract: Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages. Information

Substance Name: Interferon Type II

Registry Number: 82115-62-6

Grant and Affiliation Information for Quantitative expansion of ES cell-derived myeloid progenitors capable of differentiating into macrophages.

AFFILIATION: Division of Endocrinology, Metabolism and Gerontology, Department of Medicine and Graduate Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305-5103, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: R01 HL076746-03

ACRONYM: HL

MEDLINETA: J Leukoc Biol

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