Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues.

Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Research Abstract Details 

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Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Abstract Text:

S Kakiuchi,S Yasuda,R Yamazaki,Y Teshima,K Kanda,R Kakiuchi,K Sobue,

Although calmodulin is generally regarded as a soluble protein, a considerable amount of calmodulin activity was found to be associated with particulate fractions of mammalian tissues after an extensive washing of the particulate fraction with EGTA. Identity of this particle-bound and EGTA-nonextractable form of calmodulin with soluble calmodulin was established recently (Sobue, K., Yamazaki, R., Yasuda, S., & Kakiuchi, S. (1981) FEBS Lett. 129, 215-219). The particle-associated calmodulin activity was latent to some extent and its unmasking required the presence of nonionic detergent. We have developed an assay method for the soluble and particulate forms of calmodulin in biological samples and, by means of this method, concentrations of calmodulin in rat and bovine tissues were quantitatively determined. In the supernatant, high levels (greater than 10 microM) of calmodulin were found in the testis, pituitary gland, and various areas of brain, intermediate levels (5-10 microM) in the liver, kidney, and spleen. Particulate fractions contained 10-50% of the total calmodulin contents in the tissues. Human erythrocytes contained (2.5 +/- 0.2) microM calmodulin, or (14 +/- 0.9) X 10(4) calmodulin molecules per cell.

Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Publishing Authors By Initials

S Kakiuchi,S Yasuda,R Yamazaki,Y Teshima,K Kanda,R Kakiuchi,K Sobue,

For similar urogenital system: genitalia: genitalia, male: testis research abstracts see: urogenital system: genitalia: genitalia, male: testis research

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Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 1041-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 1982

Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Keywords Mesh Terms:

KEYWORDS: Testis

MESH TERMS: analysis

Chemical & Substance for Abstract: Quantitative determinations of calmodulin in the supernatant and particulate fractions of mammalian tissues. Information

Substance Name: Calmodulin

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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