Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay.

Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay. Research Abstract Details 

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Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay. Abstract Text:

K Hirata,M Kobayashi,K Miyamoto,T Hoshi,M Okazaki,Y Miura,

A radioimmunoassay has been developed for the quantitative determination of small amounts of vinblastine in tissue cultures of CATHARANTHUS ROSEUS (L.) G. Don. Antibody was obtained by the immunization of rabbits against a conjugate of vinblastine with bovine serum albumin. The antibody had a high affinity (Ka = 1.2 x 10 (9)l/mol) and specificity for vinblastine. The usable range of standard curve for assay was 0.5-10 ng/ml. Crude alkaloid extracts of tissue cultures could be assayed and many samples could be processed in one time. It was found that the vinblastine contents of multiple shoot cultures were lower than that of intact plants but much higher than that of callus cultures.

Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay. Publishing Authors By Initials

K Hirata,M Kobayashi,K Miyamoto,T Hoshi,M Okazaki,Y Miura,

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Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Planta medica

VOLUME: 55

Page Numbers: 262-4

Journal Abbreviation: Planta Med.

ISSN: 0032-0943

DAY: 30

MONTH: Jun

YEAR: 1989

Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay. Information

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LANGUAGE: eng

NlmUniqueID: 66751

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Grant and Affiliation Information for Quantitative Determination of Vinblastine in Tissue Cultures of Catharanthus roseus by Radioimmunoassay.

AFFILIATION: Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University, 1-6, Yamadaoka, Suita, 565, Japan.

Country: Germany

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MEDLINETA: Planta Med

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