Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography.

Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Research Abstract Details 

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Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Abstract Text:

T Beppu,Y Seyama,T Kasama,T Yamakawa,

Individual non-sulfated bile acids and sulfated lithocholic acid in serum were determined by mass fragmentography. A hexafluoroisopropyl ester-trifluoroacetyl derivative of bile acid was prepared by the method of Imai et al. (J. Chromatogr. 120, 181, 1976). Deuterium labeled deoxycholic acid was used as an internal standard monitoring at m/z 623. Lithocholic acid, deoxycholic acid, chenodeoxycholic acid, ursodeoxycholic acid, and cholic acid were determined by monitoring the intensities of m/z 622, m/z 620, m/z 620, m/z 620, ad m/z 618, respectively. A serum sample of 200 microliters including 500 ng of internal standard was hydrolyzed with strong alkali, then acidified to pH 1 with 2N HCl under cooling on ice, and extracted with diethyl ether immediately. Ether extracts were derivatized without further purification. Besides this assay of non-sulfated bile acids, total serum lithocholic acid including the sulfated form was determined as follows: extraction was performed after mixing the acidified (pH 1 with 2 N HCl) hydrolysate with ether and incubation at 40 degrees C for 2 h. Bile acid peaks in the mass fragmentogram were not affected by other materials in these serum extracts. The average values of individual non-sulfated bile acids in sera from healthy fasting subjects (n=15) were as follows: lithocholic acid, 0.049 micrograms/ml; deoxycholic acid, 0.462 micrograms/ml; chenodeoxycholic acid, 0.671 micrograms/ml; ursodeoxycholic acid, 0.070 icrograms/ml; and cholic acid, 0.217 micrograms/ml. Total lithocholic acid (non-sulfated and sulfated) in sera was 0.166 microgram/ml.

Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Publishing Authors By Initials

T Beppu,Y Seyama,T Kasama,T Yamakawa,

For similar polycyclic compounds: steroids: bile acids and salts: cholic acids: deoxycholic acid: ursodeoxycholic acid research abstracts see: polycyclic compounds: steroids: bile acids and salts: cholic acids: deoxycholic acid: ursodeoxycholic acid research

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Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 89

Page Numbers: 1963-73

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1981

Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Keywords Mesh Terms:

KEYWORDS: Ursodeoxycholic Acid

MESH TERMS: blood

Chemical & Substance for Abstract: Quantitative determination of individual non-sulfated bile acids and sulfated lithocholic acid in serum by mass fragmentography. Information

Substance Name: Deoxycholic Acid

Registry Number: 83-44-3

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Country: JAPAN

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MEDLINETA: J Biochem

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