Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena.

Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena. Research Abstract Details 

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Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena. Abstract Text:

Vadim Zinchuk,Olga Zinchuk,Teruhiko Okada,

Quantitative colocalization analysis is an advanced digital imaging tool to examine antigens of interest in immunofluorescence images obtained using confocal microscopes. It employs specialized algorithms to estimate the degree of overlap of fluorescence signals and thus enables acquiring important new information not otherwise obtainable using qualitative approaches alone. As raw confocal images have high levels of background, they should be prepared to become suitable for reliable calculation of colocalization coefficients by correcting it. We provide concise theoretical basis of quantitative colocalization analysis, discuss its limitations, and describe proper use of the technique. The use of quantitative colocalization analysis is demonstrated by studying bile salt export pump and multidrug resistance associated protein 2 in the liver and major basic protein and platelet activating factor receptor antigens in conjunctiva. The review is focused on the applicability and correct interpretation of the results of colocalization coefficients calculations.

Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena. Publishing Authors By Initials

V Zinchuk,O Zinchuk,T Okada,

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Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta histochemica et cytochemica

VOLUME: 40

Page Numbers: 101-11

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ISSN: 0044-5991

DAY: 30

MONTH: Aug

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Quantitative colocalization analysis of multicolor confocal immunofluorescence microscopy images: pushing pixels to explore biological phenomena.

AFFILIATION: Department of Anatomy and Cell Biology, Kochi University Faculty of Medicine.

Country: Japan

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MEDLINETA: Acta Histochem Cytochem

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