Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays.

Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Research Abstract Details 

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Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Abstract Text:

Sundhar Ramalingam,Peter Honkanen,Lynn Young,Tsutomu Shimura,John Austin,Patricia S Steeg,Satoshi Nishizuka,

Mathematical simulations of the p53-Mdm2 feedback loop suggest that both proteins will exhibit impulsive expression characteristics in response to high cellular stress levels. However, little quantitative experimental evaluation has been done, particularly of the phosphorylated forms. To evaluate the mathematical models experimentally, we used lysate microarrays from an isogenic pair of gamma-ray-irradiated cell lysates from HCT116 (p53(+/+) and p53(-/-)). Both p53 and Mdm2 proteins showed expected pulses in the wild type, whereas no pulses were seen in the knockout. Based on experimental observations, we determined model parameters and generated an in silico "knockout," reflecting the experimental data, including phosphorylated proteins.

Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Publishing Authors By Initials

S Ramalingam,P Honkanen,L Young,T Shimura,J Austin,PS Steeg,S Nishizuka,

For similar proteins: dna-binding proteins: tumor suppressor protein p53 research abstracts see: proteins: dna-binding proteins: tumor suppressor protein p53 research

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Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Cancer research

VOLUME: 67

Page Numbers: 6247-52

Journal Abbreviation: Cancer Res.

ISSN: 0008-5472

DAY: 1

MONTH: Jul

YEAR: 2007

Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984705

Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Protein p53

MESH TERMS: metabolism

Chemical & Substance for Abstract: Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays. Information

Substance Name: Proto-Oncogene Proteins c-mdm2

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for Quantitative assessment of the p53-Mdm2 feedback loop using protein lysate microarrays.

AFFILIATION: Molecular Therapeutics Program, National Cancer Institute, NIH, Bethesda, Maryland, USA.

Country: United States

AGENCY: United States NCI

GRANT: N01-CO12400

ACRONYM: CO

MEDLINETA: Cancer Res

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