Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method.

Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Research Abstract Details 

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Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Abstract Text:

Earle Stellwagen,Qian Dong,Nancy C Stellwagen,

A variation of affinity capillary electrophoresis, called the replacement ion (RI) method, has been developed to measure the binding of monovalent cations to random sequence, double-stranded (ds) DNA. In this method, the ionic strength is kept constant by gradually replacing a non-binding ion in the solution with a binding ion and measuring the mobility of binding and non-binding analytes as a function of binding ion concentration. The method was validated by measuring the binding of Li+ ions to adenosine nucleotides; the apparent dissociation constants obtained by the RI method are comparable to literature values obtained by other methods. The binding of Tris+, NH4+, Li+, Na+, and K+ to dsDNA was then investigated. The apparent dissociation constants observed for counterion binding to a random-sequence 26-base pair (bp) oligomer ranged from 71 mM for Tris+ to 173 mM for Na+ and K+. Hence, positively charged Tris buffer ions will compete with other monovalent cations in Tris-buffered solutions. The bound cations identified in this study may correspond to the strongly correlated, tightly bound ions recently postulated to exist as a class of ions near the surface of dsDNA (Tan, Z.-J., and Chen, S.-J. (2006) Biophys. J. 91, 518-536). Monovalent cation binding to random-sequence dsDNA would be expected to occur in addition to any site-specific binding of cations to A-tracts or other DNA sequence motifs. Single-stranded DNA oligomers do not bind the five tested cations under the conditions investigated here.

Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Publishing Authors By Initials

E Stellwagen,Q Dong,NC Stellwagen,

For similar organic chemicals: alcohols: glycols: propylene glycols: tromethamine research abstracts see: organic chemicals: alcohols: glycols: propylene glycols: tromethamine research

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Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biochemistry

VOLUME: 46

Page Numbers: 2050-8

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 25

MONTH: 01

YEAR: 2007

Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Information

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LANGUAGE: eng

NlmUniqueID: 370623

Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Keywords Mesh Terms:

KEYWORDS: Tromethamine

MESH TERMS: chemistry

Chemical & Substance for Abstract: Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method.

AFFILIATION: Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM061009

ACRONYM: GM

MEDLINETA: Biochemistry

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• Quantitative analysis of monovalent counterion binding to random-sequence, double-stranded DNA using the replacement ion method.