Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos.

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Abstract Text:

Li-Bing Ma,Li Yang,Yong Zhang,Jun-Wei Cao,Song Hua,Ji-Xia Li,Li-Bing Ma,Li Yang,Yong Zhang,Jun-Wei Cao,Song Hua,Ji-Xia Li,

Mitochondria are the key generators of cellular ATP, and contain extranuclear genome-mitochondrial DNA (mtDNA). In the process of nuclear transfer (NT), heteroplasmic sources of mtDNA from a donor cell and a recipient oocyte are mixed in the cytoplasm of the reconstituted embryo. Previous studies showed inconsistent patterns of mtDNA inheritance in offspring and early fetuses generated through interspecies NT. The quantitative analysis of mitochondrial RNA (mtRNA) in interspecies cloned embryos is useful for better understanding the fate of two types of mitochondria. The components of nicotinamide adenine dinucleotide (NADH) dehydrogenase were coded by both nuclear DNA (nDNA) and mtDNA. The Subunit 1 (ND-1) is one of seven NADH dehydrogenase subunits coded by mtDNA. In present study, using real-time and reverse-transcription PCR, the copy number of species-specific ND-1 mRNA was examined in goat-sheep cloned embryos of various developmental stages, and was applied to evaluate the expression pattern of species-specific mtDNA. The results of showed that (1) the expression of mtDNA derived from goat fetal fibroblast (GFF) decreased from 1-cell stage (immediately after fused) to 2-cell stage, and could not be detected from 4-cell stage onward to blastocyst stage; (2) the expression of mtDNA derived from sheep oocyte was roughly constant from 1-cell stage to the 8-cell stage, increased gradually from 16-cell stage, and sharply at morula and blastocyst stage. Moreover, we strongly argued a mechanism, that is GFF-derived mitochondria were degraded for the depression of bioenergetic functions, and then selectively eliminated during the embryogenesis of goat-sheep cloned embryos. Mol. Reprod. Dev. 75: 33-39, 2008. (c) 2007 Wiley-Liss, Inc.

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Publishing Authors By Initials

LB Ma,L Yang,Y Zhang,JW Cao,S Hua,JX Li,LB Ma,L Yang,Y Zhang,JW Cao,S Hua,JX Li,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular reproduction and development

VOLUME: 75

Page Numbers: 33-9

Journal Abbreviation: Mol. Reprod. Dev.

ISSN: 1040-452X

DAY: 1

MONTH: Jan

YEAR: 2008

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8903333

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos.

AFFILIATION: Institute of Biotechnology, Northwest A&F University, Yangling, Shaanxi, China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Reprod Dev

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Quantitative analysis of mitochondrial RNA in goat-sheep cloned embryos Related Publications

• Airway epithelium and mucus: intracellular signaling pathways for gene expression and secretion.
• Imaging diagnosis of enlarged superior ophthalmic vein.
• Lung autotransplantation technique for treating central lung cancer of upper lobe.
• Study on the relationship between opioid receptors and fetal distress.
• The clinical value of rearrangement of IgH gene and bcl-2/J(H) fuse gene in the diagnosis of orbital lymphoproliferative disorders.
• The effect of metal copper on fracture healing in chicken.
• Transforming growth factor-beta -Smad signaling pathway cooperates with NF-kappa B to mediate nontypeable Haemophilus influenzae-induced MUC2 mucin transcription.
• [Advances in research on ion-channel gating mechanism]
• [Association of dendritic cell infiltration with prognosis and lymph node metastasis of renal cell carcinoma]
• [Changes in serum concentration of podophyllotoxin after its topical application with liposome on rat skin]
• [ECG signal compression based on best basis of wavelet packets]
• [Effect of lovastatin on proliferation and extracellular matrix secretion of rat hepatic stellate cells in vitro]
• [Effects of hypoxic exposure on coordinative expression of cytochrome oxidase subunits I and IV in rat cerebral cortex]
• [Effects of nitric oxide on pleural lymphatic stomata and lymphatic drainage in the rat]
• [Evaluation of p53, p21waf1, and PCNA in the diagnosis on lung cancer]
• [Evaluation the pathological diagnostic values of koilocyte in condyloma acuminatum]
• [Gap junctions in the nervous tissue]
• [Genotyping of HIV resistant alleles in indigenous Tibetan ethnic group of China: low frequency of CCR5triangle32 and high frequency of CCR2b-64I alleles]
• [Logistic regression analysis of wedge-shaped defects in the middle-aged and elderly]
• [Molecular genetic basis of Gilbert's syndrome]
• [Observation on the effect of disinfection to HBsAg by electrolyzed oxidizing water]
• [P2X purinoceptors and their regulations]
• [Preparation of rhBMP-2/BCB reconstituted bone xenograft and assay of its osteoinductivity]
• [Selective transmission of hepatitis B virus variant with S gene mutation between HBV-carrier mothers and their infants]
• [Surgical repair of postinfarction ventricular septal rupture]
• [Surgical treatment of anomalous origin of the left coronary artery from the pulmonary artery]
• [The correlations between plasma TNF-alpha, NO, NOS levels and brain lateralization in mice]
• [The genotype analysis of glucose-6-phosphate dehydrogenase deficiency in Yunnan province]
• [The relationship of low density lipoprotein receptor gene polymorphism and hyperlipidemia]
• [Umbilical cord blood transplantation (UCBT) in thalassemia children]