Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.

Quantitation of DNA and RNA with absorption and fluorescence spectroscopy. Research Abstract Details 

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Quantitation of DNA and RNA with absorption and fluorescence spectroscopy. Abstract Text:

Quantitation of nucleic acids is a fundamental tool in molecular biology that requires accuracy, reliability, and the use of increasingly smaller sample volumes. This unit describes the traditional absorbance measurement at 260 nm and three more sensitive fluorescence techniques, as well as three microvolume methods that use fiber optic technology in specialized cells or instrumentation. These procedures allow quantitation of DNA solutions ranging from 1 pg/microl to 50 mg/ml.

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Quantitation of DNA and RNA with absorption and fluorescence spectroscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in human genetics / editorial bo

VOLUME: Appendix 3

Page Numbers: Appendix 3D

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ISSN: 1934-8258

DAY: 22

MONTH: Apr

YEAR: 2007

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LANGUAGE: eng

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Grant and Affiliation Information for Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.

AFFILIATION: UVP, Inc., Upland, California, USA.

Country: United States

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MEDLINETA: Curr Protoc Hum Genet

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