Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development.

Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Research Abstract Details 

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Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Abstract Text:

Daniel B McClatchy,Lujian Liao,Sung Kyu Park,John D Venable,John R Yates,

Large-scale proteomic analysis of the mammalian brain has been successfully performed with mass spectrometry techniques, such as Multidimensional Protein Identification Technology (MudPIT), to identify hundreds to thousands of proteins. Strategies to efficiently quantify protein expression levels in the brain in a large-scale fashion, however, are lacking. Here, we demonstrate a novel quantification strategy for brain proteomics called SILAM (Stable Isotope Labeling in Mammals). We utilized a (15)N metabolically labeled rat brain as an internal standard to perform quantitative MudPIT analysis on the synaptosomal fraction of the cerebellum during post-natal development. We quantified the protein expression level of 1138 proteins in four developmental time points, and 196 protein alterations were determined to be statistically significant. Over 50% of the developmental changes observed have been previously reported using other protein quantification techniques, and we also identified proteins as potential novel regulators of neurodevelopment. We report the first large-scale proteomic analysis of synaptic development in the cerebellum, and we demonstrate a useful quantitative strategy for studying animal models of neurological disease.

Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Publishing Authors By Initials

DB McClatchy,L Liao,SK Park,JD Venable,JR Yates,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genome research

VOLUME: 17

Page Numbers: 1378-88

Journal Abbreviation: Genome Res.

ISSN: 1088-9051

DAY: 3

MONTH: 08

YEAR: 2007

Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Information

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LANGUAGE: eng

NlmUniqueID: 9518021

Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: chemistry

Chemical & Substance for Abstract: Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development. Information

Substance Name: Proteome

Registry Number: 0

Grant and Affiliation Information for Quantification of the synaptosomal proteome of the rat cerebellum during post-natal development.

AFFILIATION: Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41 RR11823-10

ACRONYM: RR

MEDLINETA: Genome Res

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