Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction.

Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction. Research Abstract Details 

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Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction. Abstract Text:

Xinkang Wang,Mei-Yin Hsu,Thomas E Steinbacher,Thomas M Monticello,William A Schumacher,Xinkang Wang,Mei-Yin Hsu,Thomas E Steinbacher,Thomas M Monticello,William A Schumacher,

INTRODUCTION: Platelets play a key role in thrombus formation. Determination of the platelet component in a thrombus provides pathophysiological insights to the thrombotic event and aids in selecting an appropriate therapeutic intervention. In this study a sensitive and reliable method to characterize the cellular components of experimental thrombi was developed using real-time polymerase chain reaction (PCR). METHODS AND RESULTS: Vena cava thrombosis was induced by either oxidative injury to topical FeCl(2) (FeCl(2)-VT) or stenosis-limited blood flow and a hypotonic pressure stress (stasis-VT) in rats. High levels of platelets were identified in the thrombus containing vessels by real-time PCR analysis of target gene amplification using the 2(-DeltaDeltaCT) values by normalizing the data with gene expression in naive vessels and with a housekeeping gene, ribosomal protein L32. By this analysis, the levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). Enhanced platelet enrichment in FeCl(2)-VT was also confirmed qualitatively by scanning electronic microscopic analysis. In addition, real-time PCR using a panel of genes representing vascular injury, inflammation and thrombosis showed marked induction (2(-DeltaDeltaCT)>5) in MCP-1, IL-1beta, iNOS and P-selectin mRNA expression in both models. CONCLUSIONS: These data demonstrate the utility of real-time PCR to quantitate platelets and other cell components in vascular thrombosis, which may facilitate the characterization and thus therapeutic intervention of a particular thrombotic event in both preclinical animal models and clinical conditions.

Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction. Publishing Authors By Initials

X Wang,MY Hsu,TE Steinbacher,TM Monticello,WA Schumacher,X Wang,MY Hsu,TE Steinbacher,TM Monticello,WA Schumacher,

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Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Thrombosis research

VOLUME: 119

Page Numbers: 593-600

Journal Abbreviation: Thromb. Res.

ISSN: 0049-3848

DAY: 22

MONTH: 06

YEAR: 2006

Quantification of platelet composition in experimental venous thrombosis by real-time polymerase chain reaction. Information

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LANGUAGE: eng

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AFFILIATION: Department of Thrombosis Biology, Bristol-Myers Squibb Company, Pennington, NJ 08534, USA. xinkang.wang@bms.com

Country: United States

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MEDLINETA: Thromb Res

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