Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes.

Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes. Research Abstract Details 

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Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes. Abstract Text:

Takashi Kogami,Yukari Miki,Teruo Yamada,Teruo Umegaki,Makoto Nishimura,Takashi Amo,Jun Kosaka,Junzo Sasaki,

We previously conducted basic research to quantify in situ hybridization (ISH) signals in rat testes. In this experimental model, we selected ribosomal RNA (rRNA) as the hybridizable RNA in paraffin sections, since it allowed us to easily analyze ISH signals expressed with digoxygenin (DIG)-labeled probes quantitatively through "posterization" of the images. We applied this method to analyze the quantification of transcript, PERF 15 mRNA. PERF 15 is expressed specifically in the testes and localized in the rigid cytoskeletal structure of the sperm head, and has been considered to be involved in the apoptotic process of spermatogenic cells. Quantification of the signals may help to clarify the detailed function of PERF 15. We further analyzed the signals concomitant with a confocal laser scanning microscope. The peak of PERF 15 mRNA expression was found in diplotene spermatocytes, and the amount of PERF 15 mRNA was greatest in late pachytene and diplotene spermatocytes and early spermatids, followed by early pachytene spermatocytes, and then late spermatids. PERF 15 may be involved in the events leading to meiotic division, in which apoptosis is also involved. The present study may help to determine the concentration of mRNA in tissue sections.

Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes. Publishing Authors By Initials

T Kogami,Y Miki,T Yamada,T Umegaki,M Nishimura,T Amo,J Kosaka,J Sasaki,

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Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta histochemica et cytochemica

VOLUME: 39

Page Numbers: 183-92

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ISSN: 0044-5991

DAY: 22

MONTH: 12

YEAR: 2006

Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Quantification of PERF 15 mRNA in Tissue Sections from Rat Testes.

AFFILIATION: Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikatacho, Okayama 700-8558, Japan.

Country: Japan

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MEDLINETA: Acta Histochem Cytochem

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