Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity.

Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Research Abstract Details 

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Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Abstract Text:

Santosh K Ghosh,Thomas A Gerken,Keith M Schneider,Zhimin Feng,Thomas S McCormick,Aaron Weinberg,

BACKGROUND: Human beta-defensins (hBDs) are epithelial cell-derived antimicrobial and immunoregulatory cationic peptides. Our objective was to establish an analytical tool to quantify inducible hBD-2 and -3 in body fluids. METHODS: We developed sandwich ELISAs using commercially available capture and detection antibodies and determined optimal assay conditions (with 250 mmol/L CaCl(2)) to overcome masking by endogenous components of body fluids. We used recombinant hBD as calibrators and for recovery testing. RESULTS: hBD-2 and -3 detection limits were approximately 75 ng/L and approximately 3 microg/L, respectively. Mean (SD range) values in saliva samples from healthy donors (n = 60) were 9.5 (1.2-21) microg/L for hBD-2 and 326 (50-931) microg/L for hBD-3. We did not detect hBD-3 in suction blister fluid (BF; n = 10) or bronchoalveolar lavage (BAL; n = 5) from healthy participants. We detected low hBD-2 peptide concentrations in BF and BAL, 0.16 (0.03-0.32) and 0.04 (0-0.049) microg/g total protein, respectively. We observed no correlation of hBD-2 in BF and saliva or BAL and saliva from the same person. In vaginal swabs from healthy women (n = 2), mean hBD-2 and -3 concentrations were 3.42 and 103 microg/g total protein, respectively. Cervicovaginal lavage from the same women contained mean concentrations of 1.46 and 55.5 microg/g total protein. CONCLUSION: These ELISA assays can measure inducible hBD peptide concentrations in body fluids by overcoming masking effects of anionic molecules. This approach may therefore be applicable for quantifying these peptides in health and disease.

Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Publishing Authors By Initials

SK Ghosh,TA Gerken,KM Schneider,Z Feng,TS McCormick,A Weinberg,

For similar peptides: antimicrobial cationic peptides: defensins: beta-defensins research abstracts see: peptides: antimicrobial cationic peptides: defensins: beta-defensins research

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Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Clinical chemistry

VOLUME: 53

Page Numbers: 757-65

Journal Abbreviation: Clin. Chem.

ISSN: 0009-9147

DAY: 22

MONTH: 02

YEAR: 2007

Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421549

Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Keywords Mesh Terms:

KEYWORDS: beta-Defensins

MESH TERMS: analysis

Chemical & Substance for Abstract: Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Information

Substance Name: beta-defensin 3

Registry Number: 0

Grant and Affiliation Information for Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity.

AFFILIATION: Department of Biological Sciences, Case School of Dental Medicine, Cleveland, OH 44106-4905, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: R01DE16334

ACRONYM: DE

MEDLINETA: Clin Chem

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