Quantification of fibronectin matrix assembly sites using a novel ELISA assay.

Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Research Abstract Details 

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Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Abstract Text:

Mingzhe Zheng,Anthony Ambesi,Lin Yu,Paula J McKeown-Longo,

Binding of the N-terminus of fibronectin to assembly sites on the cell surface is an essential step in fibronectin fibrillogenesis. Fibronectin matrix assembly sites have customarily been quantified using an iodinated 70 kDa N-terminal fibronectin fragment. The 125I-70 K fragment is a less than ideal reagent because its preparation requires large amounts of plasma fibronectin and it has a fairly short shelf life. An additional limitation is that the cells responsible for binding the 125I-70 K cannot be quantified or identified directly but must be assessed in parallel cultures. To overcome these disadvantages, we developed an ELISA-based assay using a recombinant HA-tagged 70 K fragment. This assay allows for the simultaneous quantification and localization of matrix assembly sites on the surface of adherent cells.

Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Publishing Authors By Initials

M Zheng,A Ambesi,L Yu,PJ McKeown-Longo,

For similar proteins: blood proteins: vitronectin research abstracts see: proteins: blood proteins: vitronectin research

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Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Matrix biology : journal of the International Soci

VOLUME: 26

Page Numbers: 330-3

Journal Abbreviation:

ISSN: 0945-053X

DAY: 29

MONTH: 12

YEAR: 2006

Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9432592

Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Keywords Mesh Terms:

KEYWORDS: Vitronectin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Quantification of fibronectin matrix assembly sites using a novel ELISA assay. Information

Substance Name: Vitronectin

Registry Number: 0

Grant and Affiliation Information for Quantification of fibronectin matrix assembly sites using a novel ELISA assay.

AFFILIATION: Center for Cell Biology and Cancer Research, Albany Medical College, 47 New Scotland Avenue, Albany, New York 12208, USA.

Country: Germany

AGENCY: United States NCI

GRANT: R01 CA-69612

ACRONYM: CA

MEDLINETA: Matrix Biol

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