Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates.

Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Research Abstract Details 

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Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Abstract Text:

S S Zoghbi,Z el Rassi,R D Neumann,A Gottschalk,

The conjugation of antibodies (Ab) with DTPA in the presence of contaminating low molecular weight proteins is inefficient. The selective precipitation of Ab with 18% Na2SO4 as a preparative purification method for small aliquots of low Ab concentrations is successful in eliminating all contaminants as monitored by high performance liquid chromatography at 214 nm and in allowing IgG recoveries greater than 94%. Subsequently, this allows efficient conjugation and radiolabelling of Ab.

Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Publishing Authors By Initials

SS Zoghbi,Z el Rassi,RD Neumann,A Gottschalk,

For similar diagnosis: diagnostic techniques and procedures: diagnostic techniques, radioisotope: radioisotope dilution technique research abstracts see: diagnosis: diagnostic techniques and procedures: diagnostic techniques, radioisotope: radioisotope dilution technique research

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Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of radiation applications an

VOLUME: 14

Page Numbers: 529-32

Journal Abbreviation:

ISSN: 0883-2897

DAY: 21

MONTH: 02

YEAR: 1987

Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8611098

Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Keywords Mesh Terms:

KEYWORDS: Radioisotope Dilution Technique

MESH TERMS: standards

Chemical & Substance for Abstract: Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates. Information

Substance Name: Pentetic Acid

Registry Number: 67-43-6

Grant and Affiliation Information for Quality control and the preparative purification of low antibody concentrations for radiolabelling using bifunctional chelates.

AFFILIATION: Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, CT 06510.

Country: UNITED STATES

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MEDLINETA: Int J Rad Appl Instrum B

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