Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Research Abstract Details 

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Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Abstract Text:

Richard Josephson,Carol J Ording,Ying Liu,Soojung Shin,Uma Lakshmipathy,Araz Toumadje,Bradley Love,Jonathan D Chesnut,Peter W Andrews,Mahendra S Rao,Jonathan M Auerbach,

As the number of human embryonic stem cell (hESC) lines increases, so does the need for systematic evaluation of each line's characteristics and potential. Comparisons between lines are complicated by variations in culture conditions, feeders, spontaneous differentiation, and the absence of standardized assays. These difficulties, combined with the inability of most labs to maintain more than a few lines simultaneously, compel the development of reference standards to which hESC lines can be compared. The use of a stable cell line as a reference standard offers many advantages. A line with a relatively unchanging hESC-like gene and protein expression pattern could be a positive control for developing assays. It can be used as a reference for genomics or proteomics studies, especially for normalizing results obtained in separate laboratories. Such a cell line should be widely available without intellectual property restraints, easily cultured without feeders, and resistant to spontaneous changes in phenotype. We propose that the embryonal carcinoma (EC) line 2102Ep meets these requirements. We compared the protein, gene, and microRNA expression of this cell line with those of hESC lines and alternative reference lines such as the EC line NTERA-2 and the karyotypically abnormal hESC line BG01V. The overall expression profiles of all these lines were similar, with exceptions reflecting the germ cell origins of EC. On the basis of global gene and microRNA expression, 2102Ep is somewhat less similar to hESC than the alternatives; however, 2102Ep expresses more hESC-associated microRNAs than NTERA-2 does, and fewer markers of differentiated fates.

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Publishing Authors By Initials

R Josephson,CJ Ording,Y Liu,S Shin,U Lakshmipathy,A Toumadje,B Love,JD Chesnut,PW Andrews,MS Rao,JM Auerbach,

For similar cells: cells, cultured: tumor cells, cultured research abstracts see: cells: cells, cultured: tumor cells, cultured research

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MEDLINE DATE:

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Stem cells (Dayton, Ohio)

VOLUME: 25

Page Numbers: 437-46

Journal Abbreviation: Stem Cells

ISSN: 1066-5099

DAY: 3

MONTH: Feb

YEAR: 2007

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9304532

Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Keywords Mesh Terms:

KEYWORDS: Tumor Cells, Cultured

MESH TERMS: metabolism

Chemical & Substance for Abstract: Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research. Information

Substance Name: MicroRNAs

Registry Number: 0

Grant and Affiliation Information for Qualification of embryonal carcinoma 2102Ep as a reference for human embryonic stem cell research.

AFFILIATION: GlobalStem, Inc., 6 Taft Court, Rockville, Maryland 20850, USA. rjosephson@globalstem.com

Country: United States

AGENCY: United States NIA

GRANT: N01AG40002

ACRONYM: AG

MEDLINETA: Stem Cells

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