Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices.

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Abstract Text:

Jianying Zeng,Guo-Yuan Yang,Weihai Ying,Mark Kelly,Kiyoshi Hirai,Thomas L James,Raymond A Swanson,Lawrence Litt,

Previous neuron and glial cell culture studies of excessive poly (ADP-ribose) polymerase (PARP-1) activation found NAD(+) depletion, glycolytic arrest, and cell death that could be avoided by exogenous tricarboxylic acid cycle (TCA) metabolites, especially pyruvate (pyr). Pyruvate neuroprotection has been attributed to cytosolic NAD(+) replenishment, TCA metabolism, and antioxidant activity. We investigated the first two mechanisms in respiring cerebrocortical slices after a 1-h H(2)O(2) exposure to activate PARP-1. H(2)O(2) was followed by a 4-h recovery with oxy-artificial cerebrospinal fluid superfusion having either: (1) no glucose (glc) or pyruvate; (2) 10 mmol/L glc only; (3) 10 mmol/L pyruvate only; (4) both 10 mmol/L glc and 10 mmol/L pyruvate. Poly-ADP-ribosylation was quantified from Western blots and immunohistochemistry. Perchloric acid extracts were quantified with 14.1 T (31)P nuclear magnetic resonance spectroscopy. Just after H(2)O(2) exposure, ATP and NAD(+) decreased by approximately 50%, PCr decreased by 75%, and the ADP/ATP ratio approximately doubled. ATP and NAD(+) changes, but not PCr changes, were nearly eliminated if PARP inhibitors accompanied the H(2)O(2). Recovery with both pyruvate and glc was better than with glc alone, having higher ATP (0.161 versus 0.075, P<0.01) and PCr levels (0.144 versus 0.078, P<0.01), and higher viable cell counts in TUNEL and Fluoro-Jade B staining. Two-dimensional [(1)H-(13)C] HSQC spectra showed metabolism during recovery of (13)C glc or pyr. Pyruvate metabolism was primarily via pyruvate dehydrogenase, with some via pyruvate carboxylation. Pyruvate superfusion of PARP-injured brain slices helps replenish NAD(+) while providing metabolic fuel. Although this augments recovery, a strong antioxidant role for pyruvate has not been ruled out.

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Publishing Authors By Initials

J Zeng,GY Yang,W Ying,M Kelly,K Hirai,TL James,RA Swanson,L Litt,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

PUBMED ID PMID:

MEDLINE DATE:

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of cerebral blood flow and metabolism : of

VOLUME: 27

Page Numbers: 304-15

Journal Abbreviation: J. Cereb. Blood Flow Metab.

ISSN: 0271-678X

DAY: 24

MONTH: 05

YEAR: 2006

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8112566

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices. Information

Substance Name: Poly(ADP-ribose) Polymerases

Registry Number: EC 2.4.2.30

Grant and Affiliation Information for Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices.

AFFILIATION: Department of Anesthesia, University of California at San Francisco, 94143-0648, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM034767

ACRONYM: GM

MEDLINETA: J Cereb Blood Flow Metab

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices Related Publications

• A multi-feature and multi-channel univariate selection process for seizure prediction.
• Biochemoterrorism and adolescents.
• Continuous energy variation during the seizure cycle: towards an on-line accumulated energy.
• Dealing with rejection.
• Double standard, double jeopardy: research on health problems of adolescent women.
• Drugs affecting clearance of middle ear secretions: a perspective for the management of otitis media with effusion.
• ECG-gated MDCT of left-to-right shunt flow through a ventricular septal defect.
• Editorial: McDonald's acne.
• Ethics and research on adolescents.
• Funding adolescent medicine fellowship training.
• High-frequency oscillations in human temporal lobe: simultaneous microwire and clinical macroelectrode recordings.
• House calls?
• House staff well-being.
• Let the sun shine in: increasing access to research findings.
• Linear hypopigmentation.
• Looking back to the future. Stress in adolescents: gender differences in relationship to autoimmune disease.
• MR angiography of intracranial aneurysms: proceed, but with caution.
• Newer studies on the epidemiology of fungous infections of the feet.
• Non-adherence to post-transplant care: Prevalence, risk factors and outcomes in adolescent liver transplant recipients.
• Pyruvate improves recovery after PARP-1-associated energy failure induced by oxidative stress in neonatal rat cerebrocortical slices.
• Reducing the impact of stroke.
• Technology Insight: neuroengineering and epilepsy-designing devices for seizure control.
• The First International Collaborative Workshop on Seizure Prediction: summary and data description.
• The health of adolescents since Y1K.
• The quiddity of the head louse.
• The right to know.
• Warts in meat-cutters.
• When race matters.
• Whither [correction of Wither] "International" section?
• Whose manuscript is it, anyway?