Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver.

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Abstract Text:

K Akasaki,Y Yamaguchi,K Furuno,H Tsuji,

We previously purified and characterized a major lysosomal membrane glycoprotein (r-lamp-1) from rat liver [Akasaki et al. (1990) Chem. Pharm. Bull. 38, 2766-2770]. The present study describes the purification of another major lysosomal membrane glycoprotein (r-lamp-2) from rat liver and compares the tissue distribution of r-lamp-1 and r-lamp-2 in rats. R-lamp-2 was purified to apparent electrophoretic homogeneity from rat liver by a simple method with a protein yield of approximately 4.0 micrograms/g wet weight of liver. The purification procedure includes: preparation of tritosomal membranes, extraction of tritosomal membranes with Lubrol PX, wheat germ agglutinin (WGA)-Sepharose affinity chromatography, and monoclonal antibody-Sepharose affinity chromatography. R-lamp-2 exhibited an Mr of 96,000 on SDS-PAGE and had an acidic pI of less than 3.5. R-lamp-2 contained 52.3% carbohydrates. Its carbohydrate moieties were composed of numerous sialyl complex type N-linked oligosaccharides and small amounts of O-linked oligosaccharides. Both r-lamp-1 and r-lamp-2 were detected in all rat tissues examined by immunoblot analyses, while their apparent molecular weights differed among the tissues. Immunological quantitative analysis showed that the protein concentrations of r-lamp-2 were consistently lower than those of r-lamp-1 in all the tissues tested. There was a significant correlation with a regression coefficient of 0.86 in the tissue distribution between r-lamp-1 and r-lamp-2. A good correlation was also observed in the tissue distribution between acid phosphatase and r-lamp-2.(ABSTRACT TRUNCATED AT 250 WORDS)

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Publishing Authors By Initials

K Akasaki,Y Yamaguchi,K Furuno,H Tsuji,

For similar biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research abstracts see: biochemical phenomena, metabolism, and nutrition: metabolism: pharmacokinetics: tissue distribution research

PUBMED ID PMID:

MEDLINE DATE:

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 922-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1991

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Keywords Mesh Terms:

KEYWORDS: Tissue Distribution

MESH TERMS: metabolism

Chemical & Substance for Abstract: Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver. Information

Substance Name: Membrane Glycoproteins

Registry Number: 0

Grant and Affiliation Information for Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver.

AFFILIATION: Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein r-lamp-2 of rat liver Related Publications

• A simple chemical method for the determination of dermatan sulfate in the presence of chondroitin 4-sulfate and chondroitin 6-sulfate.
• Alcohol consumption and suicide mortality among Japanese men: the Ohsaki Study.
• Chicken ornithine transcarbamylase: purification and some properties.
• Cilnidipine lowered psychological stress-induced increase in blood pressure in a hypertensive man: a case report.
• Comparative analysis of the genomic structures and promoter activities of mouse Siaalpha2,3Galbeta1,3GalNAc GalNAcalpha2, 6-sialyltransferase genes (ST6GalNAc III and IV): characterization of their Sp1 binding sites.
• Cycling of an 85-kDa lysosomal membrane glycoprotein between the cell surface and lysosomes in cultured rat hepatocytes.
• Cycling of two endogenous lysosomal membrane proteins, lamp-2 and acid phosphatase, between the cell surface and lysosomes in cultured rat hepatocytes.
• Fucosylated Thomsen-Friedenreich antigen in alpha-anomeric configuration in human gastric surface epithelia: an allogeneic carbohydrate antigen possibly controlled by the Se gene.
• Function of NKT cells, potential anti-HIV effector cells, are improved by beginning HAART during acute HIV-1 infection.
• Genomic organization and transcriptional regulation of the mouse GD3 synthase gene (ST8Sia I): comparison of genomic organization of the mouse sialyltransferase genes.
• Involvement of organic anion transport system in transdermal absorption of flurbiprofen.
• Molecular cloning and genomic analysis of mouse GalNAc alpha2, 6-sialyltransferase (ST6GalNAc I).
• Molecular survey of Babesia microti, Ehrlichia species and Candidatus neoehrlichia mikurensis in wild rodents from Shimane Prefecture, Japan.
• Nanostructured bulk copper fabricated by accumulative roll bonding.
• PIAS3 interacts with ATF1 and regulates the human ferritin H gene through an antioxidant-responsive element.
• Phosphatidylserine specific binding protein in rat brain: purification and characterization.
• Poly(lactide) Stereocomplexes: Formation, Structure, Properties, Degradation, and Applications.
• Purification and characterization of a major kyotorphin-hydrolyzing peptidase of rat brain.
• Purification, some properties, and tissue distribution of a major lysosome-associated membrane glycoprotein (r-lamp-2) of rat liver.
• Species-specific epitopes exist on the cytoplasmic amino-terminal domain of erythrocyte band 3 protein.
• The Outcome of Gastrointestinal Stromal Tumors (GISTs) After a Surgical Resection in Our Institute.
• The Thomsen-Friedenreich antigen-related carbohydrate antigens in human gastric intestinal metaplasia and cancer.
• Transcriptional regulation of subtilisin-like proprotein convertase PACE4 by E2F: possible role of E2F-mediated upregulation of PACE4 in tumor progression.
• Transient expression of a brain/embryonic-type myosin heavy chain isoform (MIIB2) in regenerating rat liver.
• Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa.
• Transporter-mediated hepatic uptake of ulifloxacin, an active metabolite of a prodrug-type new quinolone antibiotic prulifloxacin, in rats.
• Unique mechanical properties of nanostructured metals.
• [Occupation and carpal tunnel syndrome]
• [Skin reaction induced by subcutaneous injection of LH-RH analogue]
• [Splenic marginal zone lymphoma associated with autoimmune hemolytic anemia treated with splenectomy and rituximab]