Purification of zymogen granules from monkey parotid glands.

Purification of zymogen granules from monkey parotid glands. Research Abstract Details 

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Purification of zymogen granules from monkey parotid glands. Abstract Text:

P Arneberg,E Dahl,R Hars,

A method giving highly purified zymogen granules from Macaca irus and Cercopithecus aethiops parotid glands in reported. A 0.3 M sucrose medium for homogenization was supplemented with 10 mM tris/HCl, pH 7.3, and 0.1 mM lauric acid to stabilize the fragile monkey zymogen granules. Nuclei and cell debris were sedimented at 150 times g. A "crude" zymogen granule fraction was trapped in the 1.0 M sucrose layer of a discontinuous sucrose gradient at 1000 times g. Equilibrium centrifugation in a continuous sucrose gradient gave a fraction of zymogen granules at 1.85 M sucrose. Compared to the homogenate, this fraction exhibited about two-fold increase in zymogen granule marker, whereas mitochondrial marker was reduced to 1/4 and lysosomal marker to 1/2. A low level of contamination from other cell organelles was confirmed by electron microscopic investigation.

Purification of zymogen granules from monkey parotid glands. Publishing Authors By Initials

P Arneberg,E Dahl,R Hars,

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Purification of zymogen granules from monkey parotid glands. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta pathologica et microbiologica Scandinavica. S

VOLUME: 83

Page Numbers: 389-94

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ISSN: 0365-4184

DAY: 20

MONTH: Jul

YEAR: 1975

Purification of zymogen granules from monkey parotid glands. Information

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LANGUAGE: eng

NlmUniqueID: 7508468

Purification of zymogen granules from monkey parotid glands. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

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Substance Name: Proteins

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Country: DENMARK

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MEDLINETA: Acta Pathol Microbiol Scand [A

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