Purification of monoclonal antibodies.

Purification of monoclonal antibodies. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification of monoclonal antibodies. Abstract Text:

S A Fuller,M Takahashi,J G Hurrell,S A Fuller,M Takahashi,J G Hurrell,

The uses of monoclonal antibodies as enzyme conjugates and as immunoaffinity reagents require their purification from the crude ascites fluid. This unit provides protocols for purification using protein A-Sepharose chromatography and affinity chromatography, which are superior to ammonium sulfate precipitation, gel filtration, or ion-exchange chromatography for the preparation of contaminant-free antibody.

Purification of monoclonal antibodies. Publishing Authors By Initials

SA Fuller,M Takahashi,JG Hurrell,SA Fuller,M Takahashi,JG Hurrell,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Purification of monoclonal antibodies. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Current protocols in molecular biology / edited by

VOLUME: Chapter 11

Page Numbers: Unit11.11

Journal Abbreviation:

ISSN: 1934-3647

DAY: 11

MONTH: May

YEAR: 2001

Purification of monoclonal antibodies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8908160

Purification of monoclonal antibodies. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Purification of monoclonal antibodies. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Purification of monoclonal antibodies.

AFFILIATION: Allelix Inc., Mississauga, Ontario.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Curr Protoc Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification of monoclonal antibodies Related Publications

• A changing context for life on Earth.
• A finite difference analysis of the field present behind an acoustically impenetrable two-layer barrier.
• Adiposity in women and children from transition countries predicts decreased iron absorption, iron deficiency and a reduced response to iron fortification.
• An analysis of the etiology of anemia and iron deficiency in young women of low socioeconomic status in Bangalore, India.
• Axial: an abused word?
• Cloning of hybridoma cell lines by limiting dilution.
• Conjugation of enzymes to antibodies.
• Freezing and recovery of hybridoma cell lines.
• Fusion of myeloma cells with immune spleen cells.
• Immunization of mice.
• Immunoblotting and immunodetection.
• Immunoblotting and immunodetection.
• Immunoblotting and immunodetection.
• Iron absorption in young Indian women: the interaction of iron status with the influence of tea and ascorbic acid.
• Iron fortification: its efficacy and safety in relation to infections.
• Iron metabolism in heterozygotes for hemoglobin E (HbE), alpha-thalassemia 1, or beta-thalassemia and in compound heterozygotes for HbE/beta-thalassemia.
• Iron-binding properties, amino acid composition, and structure of muscle tissue peptides from in vitro digestion of different meat sources.
• Linking the bioavailability of iron compounds to the efficacy of iron-fortified foods.
• Management of tetanus, using chlorpromazine and mephenesin.
• Mild riboflavin deficiency is highly prevalent in school-age children but does not increase risk for anaemia in Côte d'Ivoire.
• Oxalic acid does not influence nonhaem iron absorption in humans: a comparison of kale and spinach meals.
• Preparation of mouse feeder cells for fusion and cloning.
• Preparation of myeloma cells.
• Production and shelf stability of multiple-fortified quick-cooking rice as a complementary food.
• Prototype system for enhancement of frontal chest radiographs using eigenimage processing.
• Purification of monoclonal antibodies.
• The Vascularisation of Cartilage.
• Vagolysis.