Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA).

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Abstract Text:

R Sting,K Westphal,

Cell wall antigens from Coxiella burnetti were extracted by heat, ultrasonication, phenol, SDS or sodium deoxycholate. These antigens were subsequently purified by gel-chromatography yielding molecular weights of 700 and 270 KD. Using gel-chromatographical purification of antigens extracted by heat, ultrasonication or phenol Coxiella antigens nearly devoid of contaminations could be obtained. Of these highly purified antigens heat extracted antigen was treated with proteases, lysozyme or metaperiodate exemplarily. These treatments did neither destroy nor change its antigenic activity, except for the treatment with metaperiodate reducing the antigenic activity significantly. In further experiments heat extracted and gel-chromatographically purified Coxiella antigen was used for testing bovine sera in ELISA.

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Publishing Authors By Initials

R Sting,K Westphal,

For similar female research abstracts see: female research

PUBMED ID PMID:

MEDLINE DATE:

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 40

Page Numbers: 690-6

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Dec

YEAR: 1993

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 331325

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Keywords Mesh Terms:

KEYWORDS: Female

MESH TERMS: veterinary

Chemical & Substance for Abstract: Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA). Information

Substance Name: Antigens, Surface

Registry Number: 0

Grant and Affiliation Information for Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA).

AFFILIATION: Staatliches Tierärztliches Untersuchungsamt Stuttgart, Germany.

Country: GERMANY

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zentralbl Veterinarmed B

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay ELISA Related Publications

• Amebiasis with pulmonary involvement.
• Continuous terlipressin infusion in patients with septic shock: less may be best, and the earlier the better?
• Cyclooxygenase-2 inhibition and increased arterial vasoconstriction to vasopressin: What is the link?
• Epidemiology of Epidemic Keratoconjunctivitis.
• Exogenous adrenomedullin prevents and reverses hypodynamic circulation and pulmonary hypertension in ovine endotoxaemia.
• Experiences of Somatonorm in Sweden.
• Fleet leaders' attitudes about subordinates' use of mental health services.
• Hemodynamic effects of thoracic epidural analgesia in ovine hyperdynamic endotoxemia.
• Hollow mandrin facilitates external ventricular drainage placement.
• Is short stature a psychosocial handicap?
• LIM-homeodomain proteins Lhx1 and Lhx5, and their cofactor Ldb1, control Purkinje cell differentiation in the developing cerebellum.
• Living at risk: concealing risk and preserving hope in huntington disease.
• New frontiers in health education.
• Optimal perfusion of an intact ovary as a prerequisite for successful ovarian cryopreservation.
• Preparation and characteristics of corticosteroid-binding globulin (CBG, transcortin).
• Pulmonary resection for metastatic sarcoma.
• Purification of Coxiella burnetii antigen by gel-chromatography for use in enzyme-linked immunosorbent assay (ELISA).
• Role of adenosine triphosphate-sensitive potassium channel inhibition in shock states: physiology and clinical implications.
• Sensors, medical image and signal processing. Findings from the section on sensor, signal and imaging informatics.
• Ten years of vasopressin research in septic shock: constant dripping wears the stone.
• The Problem of the Gonococcus Carrier.
• [Immunochemistry of O-antigens of Enterobacteriaceae. IV. Analysis of the sugar constituents of Escherichia O-antigens.]
• [Methods for analysis of amniotic fluid for diagnosis of the fetal condition in Rh-incompatibility]
• [Not Available.]
• [Not Available.]
• [On the immunochemistry of the O-antigen of Enterobacteriaceae. VI. Comparison of the sugar components of polysaccharides from S and R forms of Salmonella.]
• [Perinatal and neonatal mortality and morbidity in newborn infants up to 1,000 g]
• [Reliability and responsiveness of the German version of the Disabilities of the Arm, Shoulder and Hand questionnaire (DASH).]
• [Salivary secretion and gingival hyperplasia in diphenyl hydantoin-treated guinea pigs]
• [Specificity of calcium determination using tetracycline]