Purification of a skeletal growth factor from human bone.

Purification of a skeletal growth factor from human bone. Research Abstract Details 

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Purification of a skeletal growth factor from human bone. Abstract Text:

J R Farley,D J Baylink,

A skeletal growth factor was isolated and purified from demineralized human bone matrix. A dose of 6 micrograms/mL of the purified factor significantly increased the proliferation rate of embryonic chick bone cells in serum-free culture (292% of controls, p less than 0.0001) but had no effect on embryonic chick skin cells plated at the same initial density. The factor is sensitive to inactivation by trypsin and urea, but not by collagenase, 20% butanol, or 1% mercaptoethanol. It is also resistant to inactivation by heat (stable for 15 min at 75 degrees C) and extremes of pH (stable for 30 min at 4 degrees C from pH 2.5 to 10.0). Purification of the active factor by selective heat and acid precipitations, molecular sieve column chromatography, and preparative polyacrylamide gel electrophoresis provided a material that was homogeneous by the criteria of high-pressure liquid chromatography, polyacrylamide gel electrophoresis, and isoelectric focusing. The apparent molecular weight is 83 000. The purified factor increases bone cell proliferation at doses comparable to other mitogens: 0.3 microgram/mL (3.6 nM) significantly increases DNA synthesis to 231% of controls (p less than 0.001). The purified factor was also active on cultured embryonic chick bones, enhancing the growth rate of tibiae and femurs, as measured by increased dry weight (185% of controls, p less than 0.025) and [3H]proline incorporation (164% of control, p less than 0.001), respectively.

Purification of a skeletal growth factor from human bone. Publishing Authors By Initials

JR Farley,DJ Baylink,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Purification of a skeletal growth factor from human bone. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biochemistry

VOLUME: 21

Page Numbers: 3502-07

Journal Abbreviation: Biochemistry

ISSN: 0006-2960

DAY: 6

MONTH: Jul

YEAR: 1982

Purification of a skeletal growth factor from human bone. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370623

Purification of a skeletal growth factor from human bone. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification of a skeletal growth factor from human bone. Information

Substance Name: Microbial Collagenase

Registry Number: EC 3.4.24.3

Grant and Affiliation Information for Purification of a skeletal growth factor from human bone.

AFFILIATION:

Country: UNITED STATES

AGENCY: United States NIDCR

GRANT: DE 02600

ACRONYM: DE

MEDLINETA: Biochemistry

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