Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304.

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Research Abstract Details 

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Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Abstract Text:

Pazilat Bahti,Shunmei Chen,Yang Li,Neil Shaw,Xuejun Zhang,Min Zhang,Chongyun Cheng,Gaojie Song,Jie Yin,Hua Zhang,Dongsheng Che,Abdulla Abbas,Hao Xu,Bi Cheng Wang,Zhi Jie Liu,Pazilat Bahti,Shunmei Chen,Yang Li,Neil Shaw,Xuejun Zhang,Min Zhang,Chongyun Cheng,Gaojie Song,Jie Yin,Hua Zhang,Dongsheng Che,Abdulla Abbas,Hao Xu,Bi Cheng Wang,Zhi Jie Liu,

A 10.5 kDa non-Pfam hypothetical protein, AF1514, from the hyperthermophilic archaeon Archeoglobus fulgidus has been overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 2.09 A resolution and a data set was collected at 100 K using Cu Kalpha radiation from a rotating-anode X-ray source. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 49.27, c = 106.61 A. The calculated Matthews coefficient was 3.16 A(3) Da(-1), suggesting the presence of one molecule in the asymmetric unit.

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Publishing Authors By Initials

P Bahti,S Chen,Y Li,N Shaw,X Zhang,M Zhang,C Cheng,G Song,J Yin,H Zhang,D Che,A Abbas,H Xu,BC Wang,ZJ Liu,P Bahti,S Chen,Y Li,N Shaw,X Zhang,M Zhang,C Cheng,G Song,J Yin,H Zhang,D Che,A Abbas,H Xu,BC Wang,ZJ Liu,

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Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 64

Page Numbers: 91-3

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 18

MONTH: 01

YEAR: 2008

Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304. Information

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LANGUAGE: eng

NlmUniqueID: 101226117

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Grant and Affiliation Information for Purification, crystallization and preliminary crystallographic analysis of the non-Pfam protein AF1514 from Archeoglobus fulgidus DSM 4304.

AFFILIATION: College of Life Science and Technology, Xinjiang University, Urumqi 830046, People's Republic of China.

Country: England

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MEDLINETA: Acta Crystallogr Sect F Struct

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