Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid.

Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Research Abstract Details 

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Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Abstract Text:

M Umeno,M Anai,M Sasaki,Y Takagi,

recBC DNase of Escherichia coli has been purified from the transformant, HB101/pFS11-04 (recB+ recC+), by successive ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-150 gel filtration, hydroxyapatite chromatography, DNA cellulose affinity chromatography, and second DEAE-cellulose chromatography. The purified enzyme was obtained in an overall yield of 3%. The enzyme protein appeared as a single pure component on native polyacrylamide gel electrophoresis. The purified enzyme was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional electrophoresis. The results show that recBC DNase consists of two nonidentical subunits with molecular weights of 125,000 and 135,000, and isoelectric points of 5.6 and 5.7, respectively.

Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Publishing Authors By Initials

M Umeno,M Anai,M Sasaki,Y Takagi,

For similar genetic structures: plasmids research abstracts see: genetic structures: plasmids research

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Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 98

Page Numbers: 681-5

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1985

Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Keywords Mesh Terms:

KEYWORDS: Plasmids

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid. Information

Substance Name: exodeoxyribonuclease V, E coli

Registry Number: EC 3.1.11.5

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Country: JAPAN

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MEDLINETA: J Biochem

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