Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299.

Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Research Abstract Details 

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Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Abstract Text:

M Kobayashi,K Matsuda,

Dextransucrase [EC 2.4.1.5] activity from cell-free culture supernatant of Leuconostoc mesenteroides NRRL B-1299 was purified by (NH4)2SO4 fractionation, adsorption on hydroxyapatite, chromatography on DEAE-cellulose and gel filtration on Sephadex G-75. The extracellular enzyme was separated into two principal forms, enzymes I and N, and the latter was shown to be an aggregated form of the protomer, enzyme I. Enzymes I and N were both electrophoretically homogeneous and their relative activities reached 820 and 647 times that of the culture supernatant, respectively. On sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis, enzyme N dissociated into the protomer enzyme I, with a molecular weight of 48,000. Enzyme I was gradually converted into enzyme N upon aging, and this conversion was stimulated in the presence of NaCl. The optimum pH and temperature of enzyme I activity were pH 6.0 and 40 degrees, respectively, while those of enzyme N were pH 5.5 and 35 degrees. The Km values of enzymes I and N were 13.9 and 13.1 mM, respectively. Ca2+, Mg2+, Fe2+, and Co2+ stimulated the activity of enzyme N, and EDTA showed a potent inhibitory effect on this enzyme. Moreover, the activity of enzyme N was more effectively stimulated by exogenous dextrans as compared with enzyme I.

Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Publishing Authors By Initials

M Kobayashi,K Matsuda,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 79

Page Numbers: 1301-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1976

Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and properties of the extracellular dextransucrase from Leuconostoc mesenteroides NRRL B-1299. Information

Substance Name: Sucrase

Registry Number: EC 3.2.1.48

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Country: JAPAN

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MEDLINETA: J Biochem

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