Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis.

Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Research Abstract Details 

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Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Abstract Text:

T Nakayama,M Irikura,Y Kurogi,H Matsuo,

Bacillus subtilis sporulating cells at stage III were fractionated into mother cell and forespore fractions by means of a lysozyme-detergent method. Three forms of DNA-dependent RNA polymerase enzymes, termed M sigma, F sigma, and F delta, in addition to core enzyme (alpha 2, beta', and beta) have been purified from the cell fractions. Enzymes M sigma and F sigma are present in the mother cell and forespore, respectively, and contain sigma factor of 55,000 daltons in addition to the core subunits. On the other hand, enzyme F delta is present specifically in the forespore and contains delta 1 factor of 28,000 daltons instead of the sigma factor. The amount of RNA polymerase in the forespore is about twice that in the mother cell. The enzymes M sigma and F sigma also differed in their elution profiled from DEAE-cellulose columns and in their heat stabilities indicating that the two sigma-containing holoenzyme forms may be different in their structural properties. The enzyme F delta transcribed B. subtilis DNA about 1.6 times more actively than enzyme F sigma, and the enzymes M sigma and F sigma transcribed the DNA about 2.2 times more actively than did core enzyme.

Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Publishing Authors By Initials

T Nakayama,M Irikura,Y Kurogi,H Matsuo,

For similar genetic processes: gene expression: transcription, genetic research abstracts see: genetic processes: gene expression: transcription, genetic research

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Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 89

Page Numbers: 1681-91

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1981

Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Keywords Mesh Terms:

KEYWORDS: Transcription, Genetic

MESH TERMS: enzymology

Chemical & Substance for Abstract: Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis. Information

Substance Name: DNA-Directed RNA Polymerases

Registry Number: EC 2.7.7.6

Grant and Affiliation Information for Purification and properties of RNA polymerases from mother cells and forespores of sporulating cells of Bacillus subtilis.

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Country: JAPAN

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MEDLINETA: J Biochem

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