Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato.

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Abstract Text:

Y Tanaka,I Uritani,

L-Phenylalanine ammonia-lyase (PAL) activity was developed in response to cut injury in sweet potato root tissue. The enzyme was purified from tissue incubated for 1 day after slicing by ammonium sulfate fractionation, column chromatographies on L-phenylalanyl Sepharose 4B, phosphocellulose. Sephadex G-200 and Sepharose 6B and preparative polyacrylamide gel electrophoresis. The molecular weight and sedimentation coefficient were estimated to be 285,000 to 320,000 and 11.6 to 11.9 S, respectively. Electrophoresis on sodium dodecyl sulfate-polyacrylamide gel yielded a single stained protein band which corresponded to a subunit weight of 80,000. Thus, the enzyme seems to be composed of four subunits of the same size. Neither L-tyrosine nor D-phenylalanine served as a substrate. Two Km values for the PAL were observed above and below 30 micrometers at various temperatures and were lower than those for PALs of other plants. The slope of the Arrhenius plot had a discontinuity at 17 degrees C. The values of activation energy were calculated to be 15,000 cal and 19,000 cal above and below 17 degrees C, respectively. Similar discontinuities were also observed in the effect of temperature on the Km values and the Hill coefficients. Negative cooperativity was observed at 10 degrees C (n = 0.83), but was not marked above 20 degrees C (n = 0.94).

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Publishing Authors By Initials

Y Tanaka,I Uritani,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

PUBMED ID PMID:

MEDLINE DATE:

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 963-70

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Apr

YEAR: 1977

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato. Information

Substance Name: Phenylalanine Ammonia-Lyase

Registry Number: EC 4.3.1.5

Grant and Affiliation Information for Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato Related Publications

• 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase in sweet potato roots.
• Characterisation of kilo electron volt neutron fluence standard with the 45Sc(p,n)45Ti reaction at NMIJ.
• Development of the fast neutron standard using a Be({alpha},n) reaction at the National Metrology Institute of Japan.
• ELECTRON TRANSPORT SYSTEM IN CERATOCYSTIS FIMBRIATA.
• Enzymatic synthesis of a beta-hydroxy-beta-methylglutaric acid-derivative by a cell-free system from sweet potato with black rot.
• Genes for a nuclease and a protease are involved in the drastic decrease in cellular RNA amount in fission yeast cells during nitrogen starvation.
• Glucose-6-phosphate dehydrogenase from sweet potato: substrate-induced change in the sedimentation coefficient of the enzyme.
• Immunochemical studies on fluctuation of phenylalanine ammonia-lyase activity in sweet potato in response to cut injury.
• Isolation and characterization of acid invertase inhibitor from sweet potato.
• O-DIPHENOL OXIDASES IN SWEET POTATO INFECTED BY THE BLACK ROT FUNGUS.
• Partial purification and characterization of L-lactate dehydrogenase isozymes from sweet potato roots.
• Phenylalanine ammonia-lyase in sliced sweet potato roots.
• Phenylalanine ammonia-lyase in sweet potato roots: inhibition by phenylpropanoids.
• Purification and characterization of pyruvate decarboxylase from sweet potato roots.
• Purification and properties of o-diphenol oxidases in sweet potato.
• Purification and properties of phenylalanine ammonia-lyase in cut-injured sweet potato.